The Study On The Relationship Between Survivin Promoter SNP And The Colorectal Cancer

Objective: To investigate the association of the survivin genepromoter-31G/C,-141G/C,-241T/C single nucleotide polymorphism (SNP)polymorphism of Han population in Lu Zhou City of Sichuan Province ofChina and the susceptibility of colorectal cancer, also analysis the relationshipbetween these SNPs polymorphism and the clinic pathological parameter, andthe correlation between these SNPs polymorphism and the resistant relatedmarks of colorectal cancer patients. Methods: Randomly selected206cases ofHan Chinese patients with colorectal cancer cases and similar age-sexcomposition of129healthy Han Chinese as the control group from Sichuan LuZhou Medical College Hospital in2009-2011, peripheral venous bloodcollected, extracted genomic DNA from blood using method of phenol-chloroform-isoamyl alcohol, amplified the target gene fragment using PCR,using restriction fragment length polymorphism (RFLP), non-denaturingpolyacrylamidegel electrophoresis and silver staining to determine the genotypeof survivin gene-31G/C,-141G/C,-241T/C. Detailed collected clinical andpathological data of patients with colorectal cancer (tumor site, gross type,histological type, degree of differentiation, depth of invasion, lymph nodemetastasis); Collected colorectal tumor tissues from case group, detectedresistance-associated markers of the tumor by immunohistochemical technique.Using binomial distribution statistical analyzed the difference between genotype distribution of survivin gene-31G/C,-241T/C in Lu Zhou Hanpopulation and the global data published with the NCBI database; Usingchi-square test analyzed the correlation of SNP polymorphism with the risk ofcolorectal cancer and with clinical pathological parameters; Using rank sum testanalysis-31G/C genotype and tumor resistance-associated markers; Allanalyzes were performed using SPSS18.0software, with statistical significance(P<0.05), the odds ratio(OR) and95%confidence interval(95%CI)representative of the association of genotype and the allele with risk ofcolorectal cancer. Results:(1)-31G/C and-241T/C fund gene polymorphismboth in case group and control group,-141G/C did not detect the G allele;Control group-31G/C allele frequencies in our research and the published datain the NCBI database of people around the world, Asians, Europeans, Africansare significant different (P <0.05),there is no significant difference between-241T/C allele frequencies in our research and the published data in the NCBIdatabase of people around the world(P＞0.05).(2) There is significantdifference of-31G/C CC and the GG+GC genotype, the GG and CC+GCgenotype, the C and G allele frequency between case group and control group(P=0.002, P=0.026). CC genotype associated with risk of colorectal cancer(OR=2.129,95%CI=1.303-3.479). G allele is a protective factor for colorectalcancer. There are no significant differences of-241T/C genotypes and allelefrequencies between case group and control group, and there is no relationshipbetween-241T/C and colorectal cancer risk.(3) There is no relationshipbetween-31G/C and colorectal cancer clinicopathological parameters, includes occurrence site (rectum, colon), histological type (adenocarcinoma, mucinousadenocarcinoma), differentiation (high, middle-low), depth of invasion(non-layer, layer), lymph node metastasis (no, yes).(4)-31G/C genotype foundno correlation with tumor resistance-related markers expression. Conclusion:(1)-31G/C allele frequencies in Lu Zhou Han population and the publisheddata in the NCBI database of people around the world, Asians, Europeans,Africans are significant different; There is no significant difference between-241T/C allele frequencies in Lu Zhou Han population and the published datain the NCBI database of people around the world.(2) There is significantdifference of survivin promotor-31G/C CC and the GG+GC genotype, the GGand CC+GC genotype, the C and G allele frequency between case group andcontrol group. CC genotype associated with risk of colorectal.G allele is aprotective factor for colorectal cancer.(3) There is no significant difference ofsurvivin promotor-241T/C genotypes and allele frequencies between casegroup and control group, and there is no relationship between-241T/C andcolorectal cancer risk (4) There is no relationship between-31G/C andcolorectal cancer clinicopathological parameters, includes occurrence site(rectum, colon), histological type (adenocarcinoma, mucinous adenocarcinoma),differentiation (high, middle-low), depth of invasion (non-layer, layer), lymphnode metastasis (no, yes).(5) Survivin promotor-31G/C genotype found nocorrelation with tumor resistance-related markers expression.