Study On The Relativity Of Liver Damage, NF-κB And TNF-α After Extraintestinal Rotavirus In New-born Mice

Objective:Through establishing the new-born mice model of extraintestinal rotavirusinfection, we observe their liver pathologic changes, test the level of NF-κB andTNF-αin the liver, and study the relativity of liver pathologic changes, NF-κB andTNF-α.we further discuss the mechanism of NF-κB and TNF-αresulting in liver damageafter extraintestinal rotavirus infection in new-born mice. Methods: experimental viruswere monkey rotavirus SA-11strain. Exprimental animals were BALB/C new-born micewhich were2-3days old and SPF level.48BALB/C new-born mice were randomlydivided into two groups which were virus infected group and blank matched group, andevery group have24mice. The mice of virus infected group were injected0.1mlrotavirus SA-11stain through belly cavity, while the mice of blank matched group wereinjected0.1ml physiological brine through belly cavity. Then the mice of each groupwere reared by the method of insulation, and were observed and recorded the changes ofactivity, feeding, skin condition and weight every day. Eight mice of every groupwhich were selected randomly were killed respectively on the3th day,5th day and8thday. Their liver were gained by the method of asepsis. The livers pathologic changeswere observed by optical microscope after the livers were stained byhematoxylin-eosin(HE), and the level of NF-κB and TNF-αin the liver were tested bythe method of immunohistochemistry. Results:1. Compared with the blank matchedgroup, the mice of virus infected group were lethargy, activity decreased, slow reaction,skinfold, poor appetite, loose stools，growth slowing. The mental status and otheraspects of indexes of blank matched group are all normal.2. The livers of mice of virusinfected group were damaged most severely on the3th day, alleviated on the5th day,further alleviated on the8th day and there was an overall trend of improvement from the3th day to the8th day. The livers of mice of blank matched group were all normal onthe the3th day, the5th day and the8th day.3. The expression of NF-κB and TNF-α inthe livers of the mice of virus infected group were increased greatly on the3th day, then decreased on the5th day, further decreased on the8th day and there was an overalltrend of decrease from the3th day to the8th day. The level of expression of NF-κB andTNF-α in the livers of the mice of blank matched group on the3th day, the5th day andthe8th day were all lower than the virus infected group, and there was not an overalltrend of decrease from the3th day to the8th day.4.On the3th day, the5th day and the8th day, in the livers of virus infected group there was the positive correlation betweenthe change of pathology and the expression of NF-κB, between the the change ofpathology and the expression of TNF-α, between the expression of NF-κB and TNF-α.There was not the positive correlation on the blank matched group. Conclusion:1.NF-κB and TNF-α mediate the damage of the liver of the BALB/C new-born mice afterextraintextinal rotavirus infection.2. The damage of the liver of the BALB/C new-bornmice after extraintextinal rotavirus infection is most severe on the3th day, and there isan overall trend of improvement from the5th day to the8th day.3. The expression ofNF-κB and TNF-α in the livers of the BALB/C new-born mice after extraintextinalrotavirus infection were increased greatly on the3th day and there was an overall trendof decrease from the5th day to the8th day.4. In the livers of the BALB/C new-bornmice after extraintextinal rotavirus infection there is the positive correlation between thechange of pathology, the expression of NF-κB and TNF-α.