IFN-γ Intervention Extraintestinal Rotavirus Infection In Suckling Mice And The Correlation Between The Expression Of NF-κB Research

Objective:1. Establish extraintestinal rotavirus infection in neonatal rat model toexamine intestinal RV infection of suckling mice outside the liver levels of NF-κB, andIFN-γ after intervention of liver NF-κB levels. Of NF-κB expression and the relevanceof IFN-γ.2. Observed after neonatal RV infection in the liver pathological changes, andIFN-γ after the intervention of the liver pathology.3. Pathological changes in liverNF-κB levels in the joint to investigate the different doses of IFN-γ in the process ofintervention in the role of RV infection.4. Th1/Th2imbalance through the monitoringrole of IFN-γ, IFN-γ is of clinical treatment for rotavirus enteritis provide a newapproach.Methods: Monkey rotavirus SA-11strain, repackaging,-80°C stored for use.1-2dayold animals clean used BALB/C suckling mice, the40rats were randomly divided into5groups milk, n=8, in which the control group was injected0.1ml sterile saline, andnote for the first One day, the next day with0.1ml sterile saline and injected back inthree days of suckling mice. Extraintestinal infection in the control group was injected0.1ml SA-11virus strain and recorded as the first day of the next day with0.1ml sterilesaline and injected back in three days of suckling mice. IFN-γ treatment group wasinjected0.1ml SA-11virus strain and recorded as the first day of the next day withmedium and small doses (2ug,0.2ug,0.02ug) IFN-γ in neonatal rats were injected threedays back. After infection, each group kept in isolation. Observed after inoculation onthe activities of suckling mice, diet, color, size, stool changes, with the colloidal goldmethod to collect stool antigen detection of RV. Suckling mice were sacrificed on thefifth day, specimens from liver, immune staining levels of NF-κB andhematoxylin-eosin(HE)staining observed under light microscope morphologicalchanges of the liver.Results:1. Parenteral neonatal infection group showed loss of appetite, weight gain,reduced activity, body hair, fluffy, lethargy and so on. RV stool test were positive. Intervention group in the middle dose group, the activities of suckling mice, thesituation is better appetite, weight increase, high-dose treatment group and small dosegroup neonatal weight gain, reduced activity.2. BALB/C neonatal pathological changes:the control group macroscopic liver bright red color, rich luster. Light microscope, nopathological changes in liver tissue. Liver cells were polyhedral shape, large roundnuclei, abundant euchromatin,1to several nucleoli. Extraintestinal infection in miceliver under the naked eye color dark red, swollen, light microscope, the cytoplasm ofliver cells in loose, mitochondrial swelling, vacuolar degeneration of a large number offatty change. A large number of lymphocytes in liver cells around the infiltration ofneutrophils, cell necrosis visible mitotic point. IFN-γ low-dose treatment groupcompared with parenteral liver enlargement naked eye infection did not changesignificantly compared with light infection group, the pathological changes slightlyreduced. Moderate dose of IFN-γ gross enlargement of the liver the intervention groupcompared with viral infection significantly reduced the light microscope, the livermitochondrial swelling, fatty degeneration degeneration was reduced. Someinflammatory cell infiltration, showing a small amount of spotty necrosis and a fewmitotic figures. Large doses of IFN-γ gross enlargement of the intervention groupcompared with viral liver infection increased, the light microscope, the livermitochondrial swelling, fatty degeneration was a serious change. Inflammatory cellinfiltration, spotty necrosis and large amounts can be seen mitotic.3. Parenteralinfection most visible expression of NF-κB activation in the nucleus, showed brownparticles. Medium and small dose in the intervention group stained nuclei comparedwith the scope and intensity of infection group were increased.Conclusion:1. Parenteral neonatal RV infection caused pathological changes,manifested as fatty degeneration and inflammatory cell infiltration, spotty necrosis cells.Three different doses of IFN-γ after the intervention, moderate dose group comparedwith parenteral liver pathological injury infection have reduced liver pathologicaldamage in high dose group compared with parenteral infection has increased, the smalldose group compared with the liver injury of parenteral infection pathology There wasno significant change.2. Detection of liver NF-κB showed three different doses in the intervention group compared with the expression of NF-κB increased extraintestinalinfection, but the three groups the expression of high-dose intervention group increasedmore significantly.3. Livers showed NF-κB expression, IFN-γ can stimulate theexpression of NF-κB. NF-κB expression in the appropriate context of an RV will playan active role in resistance, but beyond a certain limit will damage the liver.4.Th1/Th2drift in the secretion of Th1proinflammatory cytokines IFN-γ, at the right dose of goodfrom the body to resist the role of RV infection, high doses will damage the body.