Study Of Viremia And Extraintestinal Organs Infection In Infants With Rotavirus Diarrhea

Rotavirus is the major pathogen for infants with diarrhea in autumn and winter, which can replicate in differentiated epithelium of small intestine mucosa and result to acute and severe diarrhea. Recently, there are more and more reports for rotavirus infection in extraintestinal organs in infants with diarrhea which may cause severe consequences. Viremia was detected in infants with immunodeficiency and those with syndromes of extraintestinal infection. We have initially researched the problems that whether there was viremia of rotavirus in the common infants with rotavirus diarrhea and whether rotavirus can infect extraintestinal organs and there are our results: 1. Through initial investigation to the common infants with rotavirus diarrhea with the ripe method of nested RT-PCR, 4 was found to be positive from 60 for viremia and proved that there was viremia in common infants with rotavirus diarrhea for the first time. The results suggested that rotavirus was mainly taken by mononuclear in peripheral blood. 2. Through the clinical investigation to the organs damage, we verified that 56.28% and 36.59% of the common infants with rotavirus diarrhea was abnormal on enzyme of liver and myocardium, 7.32% and 6.28% of them was damaged obviously in liver and heart. Also, seldom of them were damaged in pancreas and blood system. To search the related factors, we found that the syndromes of diarrhea were not the direct reasons of the change of enzyme but the viremia might be one ; extraintestinal infection might have relationship to organs damage. 3. Applied the method of RT-PCRIS to detect the rotavirus infection in organs for the first time. Found rotavirus in the kidney, liver and lung of two infants who died of rotavirus infection so that proved that viremia and extraintestinal infection exited in histology and suggested rotavirus might have the ability to adsorb on and penetrate in such tissues. 4. Partly sequenced the positive PCR outcome of rotavims in serum and analysis it, the result show that it was the rotavirus sequence and serotype III; no variation was found in it. According to the clinical information and the results of pathologic anatomy, we inferred that perhaps malnutrition, repeating infection, low immunologic function and compounded with severe infection might be the one of reasons for rotavirus to infect extraintestinal organs. 5. 35% of the 60 common infants with rotavirus diarrhea appeared the syndrome of respiratory system infection and rotavirus was detected in the lung of the dead infant in our investigation, which applied the important reference for the respiratory system infection caused by rotavirus in clinical diagnose. Also, this encouraged the existence of route of respiratory tract transmission for rotavirus.