| Objective:To surveille rotavirus(RV) genotypes among neonates from 2009 to 2010 in sentinel hospital, analyse the prevalence and strains evolution of neonatal rotavirus infection, provide the theoretical basis for the effective prevention and treatment of RV infection. Methods:2026 stool specimens were collected from neonates with diarrhea or non-diarrhea hospitalized in the Neonatology Department of People’s Hospital of Xinjiang Uighur Autonomous Region during January 2009 to December 2010. RV was detected by ELISA.Clinical and general data were collected from the neonates to analyse the influence factors associated with rotavirus infection with SPSS 17.0 statistical software. The nucleic acid was extracted from rotavirus-positive specimens. Rotavirus was further genotyped for G and P types by RT-PCR. The epidemiologic information was analyzed with SPSS 17.0. Major epidemic genotype was sequencd. The sequencing results were compared with the nucleotide sequence published in GenBank, and analyzed the homology and the epidemic variation of genotype nucleotide sequence. Results:RV detection rate was 14.22% (288/2026) during the monitoring period. It was 17.78%(176/990) in 2009, and it was 10.81%(112/1036) in 2010. Nosocomial infection accounted for 60.07%(173/288). RV detection rate of diarrhea neonates(48.68%) was significantly higher than it of non-diarrhea neonates(11.42%)(χ2=160.106, P<0.001). The G/P genotyping result of 103 (36 diarrhea,67 parts non diarrhea) positive samples of 2009 and 81(35 parts diarrhea,46 non-diarrhea) positive samples of 2010 showed that:in 2009:G9 were the predominant strains(61.11%,22/36) in diarrheal neonates, followed by G3(19.45%,7/36) and the G1(2.78%,1/36), G mixed infections accounted for 8.33%(3/36), G failed to genotype accounted for 8.33%(3/36); P[6] as the predominant strains accounted for 83.33%(30/36), followed by P[8](2.78%,1/36), P mixed infection accounted for 5.56%(2/36), P failed to type accounted for 8.33%(3/36). To non-diarrheal neonates G9 were also the predominant strains accounted for 76.12%(51/67), followed by Gl(7.46%,5/67) or G3(2.99%,2/67), G mixed infection accounted for 7.46%(5/67), G failed to type accounted for 5.97%(4/67); P[6] dominated reached 85.07%(57/67), and P[8] were detected in two cases(2.99%),5 cases of mixed infection(7.46%), failed type 3 cases(4.48%). In 2010:G9 were the predominant strain accounted for 74.28%(26/35) in diarrheal neonates, followed by G3(11.43%,4/35) and G1(2.86%,1/35), G mixed infection accounted for 11.43%(4/35); P[6] were the main epidemic strains, accounted for 80%(28/35), followed by P[8](5.71%, 2/35), Pmix infection accounted for 14.29%(5/35); In non-diarrheal neonates, G9 were the predominant strains(82.61%,38/46), followed by G1(4.35%,2/46), G3(2.17%,1/46), G mixed infection accounted for 8.7%(4/46), one case was G unclassified(2.17%); P[6] led up to 80.44%(37/46), three cases were P[8](6.52%), P mixed infection were detected in 6 cases(13.04%). During the monitoring period, the most predominant strains were G9P[6] regardless of diarrheal or non-diarrheal neonates infected with RV. Sequence analysis showed that the prevalent G9 strains and P[6] strains during the monitoring period were located in two different clades, respectively. A variety of G9P[6] strains existed in diarrheal and non-diarrheal neonates at every year. The predominant strains in 2010 may evolved from the predominant strains in 2009 by gene mutation or reassortment. Different G9P[6] stains prevailed in neonates and constantly mutated and evolved. Conclusion: Rotavirus was the main pathogen causing diarrhea and nosocomial infection among neonates. G9P[6] became the dominant genotype of neonates during the monitoring period Strengthen monitoring, mad reasonable prevention and control strategies were significant for effective prevention neonatal RV infection. |
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