The Acupuncture’s Influence On The Expression Of Dopamine Receptors In Rats With Cerebral Stroke Spastic Paralysis

Objective:To explore the mechanism of acupuncture on relieving stroke spasticity by observing acupuncture treatment methods’influence on the Dopamine receptors expression of substantia nigra and cerebral cortex in spastieity model rats.Methods:40Experimental SD rats were randomly divided into blank control group, sham operation group,model group,conventional handy acupuncture group (modeling+routine handy acupuncture on Yanglingquan and Quchi), electroacupuncture group (modeling+electroacupuncture on Yanglingquan and Quchi).Use the method of suture-occlusion to prepare stroke paralysis model in rats, the conventional handy acupuncturegroup and electroacupuncture group began treatmentafter the modeling,continuous3days.Use the Nervous sysem damage symptom score and Spasticity assessment score to evaluate model and the effect of acupuncture, use the ELISA to analyse acupuncture treatment methods’influence on DA、DRD1、DRD2contents in substantia nigra and cerebralcortex which of rats with cerebral stroke spastic paralysis, use the reverse transcription-PCR to observe acupuncture treatment methods’influence on DRD1mRNA、DRD2mRNA expression of substantia nigra and cerebral cortex in spastieity model rats. Results:(1)Nervous system damage symptom score:Before treatment, compared with blank control group and sham operation group, the scores of model group and acupuncture group were higher significantly, the difference was statistically significant (p<0.01); after treatment, compared with model group, the score of acupuncture group and was decreased significantly, the difference was statistically significant (p<0.01); before and after treatment,the difference of acupuncture group was statistically significant (p<0.01); there was no significant difference between ceconventional handy acupuncture group and electroacupuncture group p>0.05).(2)Muscle tension:Before treatment, compared with blank control group and sham operation group, the muscle tension of model group and acupuncture group was higher significantly, the difference was statistically significant (p<0.01); after treatment, compared with model group, the muscle tension of acupuncture group and was decreased significantly, the difference was statistically significant (p<0.01); before and after treatment,the difference of acupuncture group was statistically significant (p<0.01); there was no significant difference between ceconventional handy acupuncture group and electroacupuncture group (p>0.05).(3)DA、DA1R、DA2R contents in cerebral cortex:Compared with blank control group and sham operation group, contents of model group were decreased significantly, the difference was statistically significant(p<0.01); compared with model group, contents of conventional handy acupuncture group were increased significantly, the difference was statistically significant (p<0.05); compared with model group, contents of celectroacupuncture group were increased significantly, the difference was statistically significant (p<0.05, p<0.01);there was no significant difference between conventional handy acupuncture group and celectroacupuncture group (p>0.05).(4)DA、DRD1、DRD2contents in substantia nigra:Compared with blank control group and sham operation group, contents of model group were decreased significantly, the difference was statistically significant (p<0.01); compared with model group, contents of conventional handy acupuncture group were increased significantly, the difference was statistically significant (p<0.05, p<0.01);compared with model group, contents i of celectroacupuncture group were increased significantly, the difference was statistically significant (p<0.01); there was no significant difference between conventional handy acupuncture group and celectroacupuncture group (p>0.05).(5)DRD1mRNA、DRD2mRNA expression in cerebral cortex:Compared with blank control group and sham operation group, expression of model group was decreased significantly, the difference was statistically significant (p<0.01); compared with model group, expression of conventional handy acupuncture group was increased significantly, the difference was statistically significant (p<0.05); compared with model group, expression of celectroacupuncture group was increased significantly, the difference was statistically significant (p<0.05, p<0.01); there was no significant difference between conventional handy acupuncture group and celectroacupuncture group (p>0.05).(6) DRD1mRNA、DRD2mRNA expression in substantia nigra:Compared with blank control group and sham operation group, expression of model group was decreased significantly, the difference was statistically significant (p<0.01); compared with model group, DRD1mRNA expression of conventional handy acupuncture group was increased significantly, the difference was statistically significant (p<0.05); but the expression of DRD2mRNA without statistical significance (p>0.05) compared with model group, expression of celectroacupuncture group was increased significantly, the difference was statistically significant (p<0.01); there was no significant difference between conventional handy acupuncture group and celectroacupuncture group (p>0.05).Conclusion:Acupuncture has a good effect on the spasticity of paralysed limbs after stroke, it’s function mechanism is may relative to ajusting the content and expression levels of DA and DA receptors in substantia nigra and cerebralcortex. There was no difference between conventional handy acupuncture and celectroacupuncture in therapy for stroke spasticity, and to provide experimental foundation for selecting onventional handy acupuncture or celectroacupuncture in clinical treatment.