| Traditional Chinese medicine (TCM) is not only the precious wealthof the Chinese nation, but also the crystallization of splendid culture ofmany thousands of years. Traditional medical treatment idea isgradually accepted by the world and becoming more and morepopular.Chinese medicine preparations are getting increasinglyattention by the international community,as the main means of clinicalmedicine of traditional Chinese medicine,with its advantages of uniquecurative effects,little side effects,etc. The ingredients of traditionalChinese medicine is very complex, it is difficult to separate all theingredients out.Usually by measuring the main ingredient to curativeeffect to control the quality of the traditional Chinese medicine (TCM).There are many methods for the determination of the maincomponent of traditional Chinese medicine, the most commonlymethods are high-performance liquid chromatography method, highperformance capillary electrophoresis method, ultravioletspectrophotometry and thin layer chromatography scanning method,derivative spectra method, near-infrared spectra method, two spectrumassociated usage and so on.The high-performance liquidchromatography (HPLC) has the most widely application scope,therelatively simple operation,high sensitivity and accuracy.At present,HPLC method achieve further automation,intelligent,andexpand its use of space. In addition, HPLC associated with capillaryelectrophoresis,thin layer chromatography, mass spectrometry,etc,canimprove the separation efficiency,shorten the analysis time,makes theapplication of HPLC plays more and more important role in morediscipline.Chlorogenic acid and Baicalin as the determination of the maincomponent of Qingkailing preparations,they often used as indicators tocontrol the quality of the preparations. Chlorogenic acid and Baicalinhave wide range of biological activity:chlorogenic acid is anti-viral,anti-bacterial, it also can increase white blood cells, nourish the liverand gallbladder, blood fat, reduce blood pressure, anti-tumor,scavenging free radicals and can raise levels of the body P~IgE, IgG,and II-7the level lowering blood pressure and the excitement of thecentral nervous system, etc.Baicalin,because of it’s broad-spectrumantimicrobial activity on a variety of Gram-positive bacteria, such aspneumococcus, staphylococcus aureuscocci, hemolytic streptococcushad certain inhibition, gram-negative bacteria,gonorrhea double thecocci, Shigella, Escherichia coli, Proteus mirabilis, etc,is called TCMantibiotics. It also has different degrees of certain inhibition in such as avariety of pathogenic skinfungi Candida albicans cocci.Qingkailingpreparations can be used for fever caused by exogenous wind heat,fever god faint, stroke hemiplegia, delirious, and for acute and chronichepatitis, hepatitis B, upper respiratory tract infection, sore throats,pneumonia, and cerebral thrombosis, cerebral hemorrhage, etc.Tetramethylpyrazine is the main ingredient in Rhizoma Chuanxiong,it has the efficacy of invigorate the circulation of qi, chills and pain ofefficacy,besides,also can improve microcirculation and cerebral blood flow, anti-platelet aggregation and the expansion of the role of the smallarteries.It also has a significant effect of vasculitis, occlusive vasculardisease, coronary heart disease, cerebral thrombosis, angina,etc.However, due to its low content of its biological products, it is difficult forus to get the measurement. Sample preparation is the key to highperformance liquid chromatography.This topic is divided into two parts, four chapters.The first part ismade of the first two chapters,mainly use the HPLC to determinate thecontent of chlorogenic acid and baicalin glycosides in differentformulations of the same manufacturer, different batches of the samedosage form Qingkailing particles and Qingkailing capsule.Choose thebest mobile phase acidity by reference to the relevant literature andtrials, the optimal gradient elution, detection wavelength, flow rate andcolumn temperature conditions. And by the linear relationship of visitsby HPLC, detection limit, precision, accuracy, stability, recovery, andcontent of the samples was measured to verify the scientific of themethod. The second part,using the latest pre-processing methods-polymer monolith solid phase micro extraction, enrichment Chuanxiongserum, extraction, elution, and follow-up its content, and thendetermined by HPLC.The results show that the method is simple, rapid,reproducible, high sensitivity and can be used for the quality control oftraditional Chinese medicine preparation. |
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