Study On Purification Process Of Cichoric Acid And Caftaric Acid In Echinacea Purpurea And Property Of Cichoric Acid

Echinace purpurea is a native north American perennial medicinal herb that has been used for many years as an agent to enhance immune system function.It is composed of four major groups of compounds:the caffeic acid derivatives(caftaric acid,cichoric acid and echinacoside), alkamides,glycoproteins,and polysaccharides.Cichoric acid is one of the most important immune active component.So far,the cichoric aicd of high purity is difficult to prepared in large quantity,so that is restricted in Pharmacology research.In the thesis,a large scale preparation of cichoric acid was studied.Meanwhile,we have studied the stability of cichoric acid in the artificial gastric juices and artificial intestinal juice by high performance liquid chromatography to examine the stability in the environment of simulation stomach and intestines way.A rapid on-line method for screening of Echinace purpurea extract for radical scavenging components was developed.We has mainly finished the following work:1.A purified process was developed for the simultaneous purification of cichoric acid and caftaric acid in Echinacea Purpurea by pH gradient Elution.This method has the following advantages: 1)Sample Pretreatment is simple;2)It is green environmental protection due to the products were purified by macroporous resin column Chromatography with water containing acid as eluent;3)it can save production cost because Recycling Utilization of reagent is achieved.2.The stability of Echinecea purpurea preparation and cichoric acid in simulated gastrointestinal tract was studied.A HPLC method was developed to determine the stability of cichoric acid in the Artificial Gastric Juices and Artificial Intestinal Juice.The research can provide the basic support for the metabolite in body for cichoric acid.The result demonstrated that cichoric acid is stable in the Artificial Gastric Juices and Artificial Intestinal Juice.And can be made into oral preparation.3.A rapid on-line screening for antioxidant activity in extract of echinecea purpurea was developed.The extract of Echinecea purpurea was separated by HPLC,and HPLC-separated analytes react postcolumn with the DPPH solution,and the induced bleaching is detected as a negative peak by an absorbance detector at 517 nm.There was the negative peak if the compound in Echinecea purpurea showing the antioxidant activity.The method can be applied to screen the active component from natural product quickly.