Polymorphism Of16Y-chromosome STR Loci In Han Ethnic Group In Hunan Population

Objective:Aim to obtain the allele frequence data and relatived genetics parameter of Han ethnic group in Hunan province, we detected16Y-STR loci of them, these information would have been used for human identification in forensic cases and paternity tests in Hunan province, this work would promoted the built of population database in Hunan province, and enrich the population genetic data information of Chinese han nationality. This study can also provide reference data for medicolegal identification in other areas and the human genetics research work.Method:1. The selection of studied samples:174health male of Han ethnic group were selected from routine paternity test cases internal of2008-2009. After consent were obtained, we collected the blood stain of them for following use.2. Genomic DNA extraction:Chelex-100and proteinase K protocol were used for genomic DNA extraction.3. PCR amplification:16Y-STRs (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635and YGATA H4) were co-amplified by AmpF1STR1Y filerTM PCR Amplification kit (ABI Applied biosystems). The PCR was performed in a9700Perkin-Elmer DNA Thermal Cycler (Applied Biosystems).4. Electrophoretic separation and gene typing:The PCR products were separated using capillary electrophoresis on an ABI3130XL PRISM_DNA Genetic Analyzer (Applied biosystems, Foster City, CA, USA). The17Y-STR results were analyzed using GeneMapper ID v.3.2(Applied biosystems, Foster City, CA, USA). Allele designations were determined by comparison of the sample fragments with those of allelic ladders provided with kit.5. Data statistics and analysis:Excel were used for data statistics, the gene diversity were calculated with formula GD=n(1-∑Pi2)/(n-1)(Pi=the frequency of allele or haplotype, n=the number of samples). ARLEQUIN3.1were used for comparation of populations. MEGA4was used to build genetic phylogeny tree.Result:1. The frequencies distribution of16Y-STR loci among0.0143-0.7143, gene diversity among0.4476(DYS438)-0.8365(DYS385). The accumulate GD=0.9998, the polymorphism of these16Y-STR loci is high, fit for the paternity identification and individual identification work in Hunan province.2. The comparation of populations indicated, there is no significant differentiation between this population and Zhejiang Han and Taiwan Han populations(P>0.05), and significant differentiation were found with Shandong Han people, Ningxia Hui people, Japanese, Korea, Qinghai Zang people, Lasa Zang people, Zang people and sala people of China, Siberia population (P<0.05).3. Genetic phylogeny tree built by MEGA4.0analysis software showed:Hunan, Taiwan, Zhejiang populations are gathered into one group, Ningxia Hui population and Qinghai Sala population are gathered into one group, Japanese and Korean are gathered into one group, Zang people from four different areas gathered into one group, which accordance with the geography distribution.Conclusion:The polymophism of16Y-STRs in Hunan Han ethnic group is high, fit for the paternity identification and individual identification work in Hunan province, this work have significant promote to the built of Y-STRdatabase in Hunan province, and provide referenceto the forensic science work of other areas.