Study On The Correlation Between SNP Typing And Methylation And Its Correlation With Violent Crimes

Abstract:Objective To establish two genotyping methods by classical denaturing gradient gel electrophoresis (DGGE) method and pyrosequencing method to genotyping rs220030, a SNP which is located in the promoter region of small nuclear ribonucleoprotein polypeptide N (SNRPN) gene. To show the advantage of pyrosequencing in SNP genotyping compared with DGGE electrophoresis. To establish a analytical technique for detecting CpG methylation status by pyrosequencing method. To investigate the feasibility of applying rs220030to parental origin allele determination. Method DGGE method was applied to genotype rs220030of97individuals from31families from Shanghai Han population, meanwhile pyrosequencing method was applied to genotype rs220030of25individuals from7families which included in97individuals above, and compare these two methods in genotyping SNP. Pyrosequencing united bisulfite conversion method was applied to detect CpG methylation status of region upstream rs220030of two famlies and investigate whether the methylation status is parental related. Results rs220030genotyping results of97individuals detected by DGGE is20C homozygote,29T homozygote,48CT heterozygote.25individuals genotyped by pyrosequencing method show the same result. The CpG methylation status of region upstream rs220030of child was similar to mother. Conclusion Compared with DGGE, pyrosequencing is more accurate, more convenient, suitable for large samples. Pyrosequencing united bisulfite conversion method can be used to detect CpG methylation status precisely. It is feasible to apply rs220030to parental origin allele determination. Abstract:Objective To investigate whether there is a relevance between the polymorphisms of SNP in the gamma-aminobutyric acid (GABA) receptor A β33subunit (GABRB3) and violent criminal action. If there is a relevance, to select the prossible SNPs in the promoter of GABRB3which may related with violent criminal action. Meanwhile, according to the result of SNP genotyping of people who have violent criminal records, to investigate the relevance between the polymorphisms of SNP in the promoter of GABRB3and epression of GABRB3. Method PCR and DNA sequencing were used to detect the polymorphisms of SNP in GABRB3in150male (Chinese Han population) Who had violent criminal records which happened in their age from16to60. Genetic information of normal healthy Chinese Han male population in the biological information database(CHB,CHS and taken both together)was used as the control group. Chi-square test was used three times to comparing differences in genotype between two group. Results rs20317> rs8179184, rs4906902loci were selected as under test SNP loci. Chi-square test show statistically significant difference in all three test in rs20317(P＜0.05). And Chi-square test show no significant difference in all three test in rs8179184and rs4906902(P>0.05). Genotyping of rs8179184is related with genotyping o." rs4906902in the same sample. Conclusion rs20317may be associated with violent criminal action. rs20317C allele may be used as the risk gene of violent criminal action. The GABRB3promoter region SNP polymorphism affect the expression of gene transcription, thereby affecting its function, may cause changes in behavior. The GABRB3gene promoter region SNP loci rs4906902and rs8179184are closely linked.