Study On Tissue Culture Of5Floribunda Roses Excellent Strains

Floribunda Roses very important ornamental value,in many parts of the world have beenwidely used in many cities also rose as the floral emblem,the reason is due to the rich theFloribunda Roses flowers normally open seasons and the Fragrance of flowers,Floribunda Rosesthe color is also very rich,very important application value in the garden.The weather iscold,Harbin and other northern areas need to be planted may be exposed to winter FloribundaRoses.Floribunda roses of YJ‘2004-2’,YJ‘2004-3’,YJ‘2004-5’,YJ‘2004-6’,YJ‘2004-8’are the fiveexcellentines of cross breeding for the laboratory after many years,which is excellent because ofexcellent traits,brightly colored,beautiful flower type,suitable for exposed to winter in the Northeasttegion and resistant to extensive management,due to the original small seedings,expect breeding alarge number of seedings to meet garden applications after the test. This study discussed the factorsthat affected the direct differentiation and callus differentiation of the FloribundaRoses,establishment of micropropagation system,compare the differences of direct differentiationand redifferentiation,explore an effective way and sustainable development,laid a bundation ofbiochnology of asexual rapid propagation of Floribunda Roses. The main findings are as follows:1.5Floribunda Roses direct differentiation of strain: The best medium for adventitious budinduction of YJ‘2004-2’,YJ‘2004-3’,YJ‘2004-5’was: MS+6-BA2.0mg·L^-1+NAA0.1mg·L^-1, theinduction rate reached95.56%,94.44%,91.33%;The best medium for adventitious bud induction ofYJ‘2004-6’,YJ‘2004-8’was:MS+6-BA2.5mg·L^-1+NAA0.01mg mg·L^-1, the induction rate reached95.56%,92.22%.2.The best medium for proliferation of YJ‘2004-2’,YJ‘2004-3’,YJ‘2004-8’ was MS+6-BA1.5mg·L^-1+NAA0.3mg·L^-1, the rate of proliferation can reach4.31,4.38,4.36;the best medium forproliferation of YJ‘2004-5’,YJ‘2004-6’ was MS+6-BA2.0mg·L^-1+NAA0.1mg·L^-1, the rate ofproliferation can reash4.34,4.16.YJ‘2004-2’,YJ‘2004-3’,YJ‘2004-5’,YJ‘2004-6’,YJ‘2004-8’inoculation of each strain in1/2MS medium and add the auxin NAA concertration of0.08mg·L^-1when in1/2MS medium and add the auxin NAA concertration of0.1mg·L^-1，the rooting results arebest and the best rooting rate was82.22%; tissue culture domesticated in vermiculite results arebest.3.5Floribunda Roses strains leaf callus culture induction medium.YJ‘2004-2’ and YJ‘2004-8’leaves callus culture was inoculated in MS+2,4-D2.0mg·L^-1+6-BA1.0mg·L^-1+NAA2.0mg·L^-1, the rates of inducation are95.56%and92.22%; The best medium for leaves callus induction ofYJ‘2004-3’. YJ‘2004-5’ and YJ‘2004-6’ is: MS+2,4-D2.0mg·L^-1+6-BA2.0mg·L^-1+NAA0.5mg·L^-1,the rate of inducation can respectively reach94.44%,93.33%,96.67%.The leaf induction rate ofcallus higher than the young stems induction rate of callus.4. Screened young stems callus differentiation medium of the five Flobunda Roses strains.YJ‘2004-2’ Floribunda Roses strains and inoculated on MS medium containing auxin TDZconcentration is6.0mg·L^-1,2,4-D concentration is0.1mg·L^-1and GA3concentration is0.5mg·L^-1,the rate can reach40.33%; YJ‘2004-3’ Floribunda Roses strains and inoculated on FloribundaRoses strains and inoculated on MS+6-BA0.5mg·L^-1+2,4-D0.5mg·L^-1+TDZ7.0mg·L^-1, the rate canreach28.33%; the best medium for young stems callus differentiation of YJ‘2004-5’isMS+6-BA0.5mg·L^-1+TDZ8.0mg·L^-1+GA30.1mg·L^-1, the rate can reach34.33%; the best mediumfor young stems callus differentiation of YJ‘2004-6’is MS+TDZ6.0,mg·L^-1, the rate can reach46.67%; YJ‘2004-8’ Floribunda Roses strains and inoculated on MS+TDZ7.0mg·L^-1+GA30.1mg·L^-1,+2,4-D0.1mg·L^-1the rate can reach35.33%.Select the young stems for callusculture’s differentiation ability higher than the leaves for callus culture.5.The best rooting medium for five Roses strain is1/2MS+NAA0.1mg·L^-1,rooting rate can beup to80%;domesticated matrix are vermiculite and peat,the survival rate of open field planting canup to80%through domestication hardening of arious strains.6.Select explants of stem segments with axillary induced buds were best, direct and rapidpropagation of callus regeneration system faster than plant breeding, induction and proliferationrates are higher.