Study On Key Technology Of Rose Tisssue Culture

It is necessary to study technology of rose tissue culture for good seed cultivation and industrialization.All work and conclusions or results are below:1 Choosing explant. Rose stem is explant. There are two proper choosing periods in a year. One is from the middle of March to the end of April. The other is October. At the former period, axillary buds on explant germinate easier, grow quicker, but contamination and browning is serious; at the later period, circumstance is contrary.2 Sterilization of explant. Sum of live rate and un-contamination rate of explant is statistic index. It revealed that soaking explant by spirit (70%,m/m) for 6 seconds and Hgch (0.1~0.2 %,m/m) for 8 minutes can sterilize explant well.3 Calculative basis of agar dosage. Put forward the viewpoint of denoting the rigidity of media by peptonization intensity(g/cm2).Ascertained that the peptonization intensity of initial media is 160-170 g/cm2,that of subculture and rooting media is 150~160 g/cm2.4 Experiment on sugar source of media. The result of comparison between sucrose, maltose and lactose showed that type K is suitable to sucrose 30g-L-1 in initial culture, 40g-L-1 in subculture and 30g-L-1 in rooting culture; type B is suitable to sucrose 40g-L-1 in initial culture, maltose 30g-L-1 in subculture and 40g-L-1 in rooting culture. Disinherited the tradition of choosing sucrose solely.5 Optimizing combination of hormone content. Experimental results showed that type K is fitting to 1.50mg-L-16-BA + 0.02mg-L-1 NAA in initial culture,1.0mg-L-16-BA + 0.1mg-L-1 NAA + 0.1mg-L-1PP333 in subculture and 0.4g-L-16-BA+0.1mg-L-1 NAA+0.2g-L-1AC in rooting culture; type B is fitting to 2.02mg-L-16-BA+0.11mg-L-1 NAA in initial culture,1.8mg-L-16-BA+0.5mg-L-1 NAA+ 0.5mg-L-1PP333 in subculture and 0.68mg-L-16-BA+0.27mg-L-1 NAA+0.40g-L-1AC in rooting culture.6 Selecting pH for special media. It showed that the proper pH of initial media for two types rose is 5.9,that of multiplying and rooting media is 5.8.7 Study on factors of media's pH. Dose type used to revision of media's pH, peptonization intensity of media, temperature, high temperature and pressure pasteurization are factors of media's pH; Sterilizing time, volume of media, content of pledget, NaCl,CaCl2,sucrose and active carbon can Iead to the lowing of MS media's pH after high temperature and pressure pasteurization; The influencing trend and extent of every factor is different obviously.8 Study on media simplification experiment. The result showed that the effect of replacing tap water by distilled water, replacing analysable sucrose by common white sugar, replacing cone bottle by general bottle on tissue culture is very little. However, it can reduce the cost of tube culture seeding.