| In this study.the objects of this study were Chinese Merino (Xinjiang Type)(ZM),crossbred population of Chinese Merino (Xinjiang Type) and German Merino(ZD), crossbred population of Chinese Merino (Xinjiang Type) and Suffolk(ZS).The microsatellite marker technology was applied to detect genetic diversity at eight microsatellite marker(MAF70, BM1824, OARHH35, OARJMP8, BMS1248, MCM38, BMS648, ETH225) in Chinese Merino (Xinjiang Type) and its hybridizationpopulation.The correlation between genetic marker and production traits were analyzed using general linear model(GLM). This study will be designed to provide experimental basis for the utilization of sheep heterosis and marker assisted selection (MAS).The results were as follows:1. According to the analysis of productive traits of Chinese Merino (Xinjiang Type) and its Crossbred Populations, the results were:The weight,weaning weight and six month weight of ZD and ZS were significantly higher than ZM (p <0.01),the wool yield of ZD and ZS were significantly lower than ZM (p<0.01),the heart girth of ZS was significantly higher than ZM (p<0.01),the fineness of ZS was significantly higher than ZM (p<0.01),the scouring yield of ZS was significantly lower than ZM (p<0.01).The two Crossbred progeny have the obvious heterosis on the the meat performance.ZS is more higher than ZD,but the Wool quality of ZS has disadvantages heterosis.2. According to the analysis about eight microsatellite markers of Chinese Merino (Xinjiang Type) and its crossbred population, the results showed the eight microsatellite markers were detected and got specific amplification.58alleles were got in all markers and average allelic number was7.3. The effective number of allele (Ne) which was the most one was9at MCM38in ZD.The Ne which was the least one was5at OARJMP8in the three populatoins.The allele number of eight microsatellite markers are more than four, can be used to analyze genetic diversity in three sheep populations. The average effective number of alleles which is eight microsatellite markers in three sheep populations respectively is5.7431,5.8248,5.6110. The average heterozygosis respectively is0.8101,0.8229,0.8192. The average polymorphism information content is0.8078,0.7982,0.7947. This result showed that genetic variation was higher, genetic polymorphism was plentiful. These microsatellite loci could be used to analyze the correlation between microsatellite marker polymorphism and productive traits in Chinese Merino (Xinjiang Type) and its crossbred population.3.The correlation between genotypes of8microsatellite markers and productive traits of three sheep populations was analyzed, the results indicated that in wool traits, the genotype CG of BMS648was higher than on scouring yield (P<0.05) on wool yield in ZM, the genotype AD of OARJMP8was higher than genotypes CE (P<0.05) on wool yield in ZD, the genotype CG of BMS1248was lower than other genotypes (P<0.05) on wool yield in ZD. The genotype CE of OARHH35was higher than genotypes AF (P<0.05) on scouring yield in ZM, the genotype BB of BMS648was higher than other genotypes (P<0.05) on scouring yield in ZM, the genotype DF of OARHH35was higher than genotypes BG (P<0.05) on scouring yield in ZD.BMS648, OARJMP8and BMS1248were regard as genetic marker of sheep wool yield traits choice, OARHH35and BMS648were regard as genetic marker of sheep scouring yield traits choice.In different growth stages of the weight, the genotype DH of BMS1248was higher than other genotypes (P<0.05) on weight in ZD.The genotype DD of ETH225was higher than genotype CC (P<0.05) on weaning weight in ZD, the genotype BD of MAF70was higher than genotype BG (P<0.05) on weaning weight in ZS.BMS1248was regard as genetic marker of sheep weight traits choice, ETH225and MAF70were regard as genetic marker of sheep weaning weight traits choice. |
