Analysis On Polymorphism Of Microsatellite Markers And Its Association With Economic Traits In Xinijang Local Goat Breeds

In this study, the study object were Xinjiang Goat and Qinggeli Cashmere Goat. The microsatellitemarker technology was applied to detect genetic diversity at seventeen microsatellite markers(BM1824，BM3033，BMS1724，BMS1788，BM6506，OarJMP8，MCM38，LSCV15，BMS1248，MAF70，BM3413，BMS2782，OarHH35，BMS6438，BM143，OarAE101，BMS835) in two local Xinjinag Goat breeds.Thecorrelation between genetic marker and production traits were analyzed using general linear model (GLM).These will benefit for the protection, development and application of goat genetic resource, application ofDNA marker related to cashmere economic traits on marker-assist-selection (MAS) breeding for two localXinjiang goat breeds in China.The results were as follows:1. According to the analysis of productive traits of Xinjiang Goat and Qinggeli Cashmere GoatPopulations, the results were:As for the fineness, Xinjiang goat fineness was significantly lower than Qinggeli cashmere goatfineness（P＜0.05）; as for the yield, Qinggeli cashmere goat yield was significantly lower than Xinjianggoat yield（P＜0.05），as for the length and rate of net cashmerethere was no significant difference betweenXinjiang two local cashmere goat breeds（P＞0.05）.2. According to the analysis of thireteen microsatellite markers of Xinjiang goat and Qinggelicashmere goat populations, the results showed that except four loci including BM143, BMS6438,OarAE101and BM835, the others are polymorphism loci.The average number of alleles among the thirteenmicrosatellite markers in two local Xinjiang goat breeds respectively is6.84and6.54; The averageeffective number of alleles respectively is4.8833and4.8087; The average heterozygosis respectively is0.7856and0.7884; The average polymorphism information content respectively is0.7532and0.7556. Thisresult showed that genetic variation was higher, and genetic polymorphism was plentiful. Thesemicrosatellite loci could be used to analyze the correlation between microsatellite marker polymorphismand productive traits in two local Xinjiang goat breeds.3. The correlation between genotypes of13microsatellite markers and economic traits of Xinjiang twolocal goat populations was analyzed, and the results indicated that as for the fineness, in Xinjiang goatpopulations, the genotype BB of BMS1788, genotype CC of OarJMP8, genotype FH of MCM38, genotypeAD of BM1824, and genotype CE of BMS1248were significantly lower than that of other genotypes (P＜0.05), and were the favorable genotypes for Xinjiang goat cashmere fineness traits. Among these genotypeFH of MCM38was significantly lower than the other microsatellite loci genotypes, so locus MCM38wasregarded as the genetic marker of Xinjiang goat fineness choice traits. In Qinggeli cashmere goatpopulations, genotype CCof MAF70, genotype BE of BM1824, genotype AE of BM3033, genotype CC ofBM6506, genotype BD and BF of BM3413, genotype BD of LSCV15, genotype BF of OarHH35andgenotype DH of MCMC38were significantly lower than that of other genotypes (P＜0.05), and were thefavorable genotypes for Qinggeli cashmere goat fineness traits. Among them genotype BD of LSCV15was significantly lower than other microsatellite loci genotypes, so locus LSCV15was regarded as thegenetic marker of Qinggeli cashmere goat fineness choice traits.As for the length, in Xinjiang goat populations, the genotype FI of MCM38, genotype AB of BM1824,genotype DF and CG of BM3033, genotype CC of BMS1724, genotype AB and AC of BM6506, genotypeFH of BMS1248, genotype AC and BD of BM3413, genotype BE and BF of LSCV15, and genotype EH ofOarHH35were significantly higher than that of other genotypes (P＜0.05), and were the favorable genotypes for Xinjiang goat cashmere length traits. Among them genotype FI of MCM38was significantlyhigher than other microsatellite loci genotypes, so locus MCM38was regarded as the genetic marker ofXinjiang goat length choice traits. In Qinggeli cashmere goat populations, genotype BD of BM1824,genotype AE of BMS1788, genotypes AA and BB of OarJMP8, and genotype CE of BM3413weresignificantly higher than other genotypes (P＜0.05）, and were the favorable genotypes for Qinggelicashmere goat cashmere length traits. Among them genotype CE of BM3413was significantly higherthan other microsatellite loci genotypes, so locus BM3413was regarded as the genetic marker of Qinggelicashmere goat length traits choice;As for the yield, among Xinjiang goat populations, the genotype DF of BMS1788, genotype BF ofMAF70, genotype BC of MCM38, genotype AB and AD of BM1824, genotype CF of BMS1248, genotypeDG of BM3413, and genotypes AB and BG of OarHH35were significantly higher than other genotypes (P＜0.05), and were the favorable genotypes for cashmere yield traits among Xinjiang goat; among these thegenotype BF of MAF70was significantly higher than other microsatellite loci genotypes, so locus MAF70was regard as the genetic marker of Xinjiang goat cashmere yield choice traits. Among Qingeli cashmeregoat populations genotype CG of BMS1788, genotype AE of MAF70, genotypes CG and DG of MCM38,genotypes BD and BE of BM1824, genotypes BC and CC of BM6506, and genotypes AC and BD ofLSCV15were significantly higher than other genotypes (P＜0.05）, and were the favorable genotypes forQinggeli cashmere goat cashmere yield traits. Among them genotype DF of BMS1788was significantlyhigher than other microsatellite loci genotypes, so locus BMS1788was regarded as the genetic marker ofQinggeli cashmere goat cashmere yield choice traits.As for the rate of the net cashmere, in Xinjiang goat populations, the genotypes AB and BB ofBMS788, genotype FH of MCM38, genotypes CD and BE of BM1824, genotype CG of BM3033,genotypes CC and DE of BMS1724, genotype AC of BM3413, genotype BB of BMS2782weresignificantly higher than other genotypes (P＜0.05）, and were favorable genotypes for Xinjiang goat rateof the net cashmere traits, and among them genotypes CC and DE of BMS1724were significantly higherthan other microsatellite loci genotypes, so loci BMS1724were regard as genetic marker of Xinjiang goatrate of the net cashmere traits choice; In Qinggeli cashmere goat populations, genotypes AD and BC ofBMS1788, genotypes CD and CE of MAF70, genotype AA of OarJMP8, genotype BE of BM3033,genotypes AB and BD of BMS1724, genotypes CE and DE of BM6506, genotype BE of BMS1248,genotypes AB and CE of BM3413, genotype DF of OarHH35were significantly higher than othergenotypes (P＜0.05), and were the favorable genotypes for Qinggeli cashmere goat net rate of cashmeretraits. Among them genotype BE of BMS1248was significantly higher than other microsatellite locigenotypes, so locus BMS1248was regard as the genetic marker of Qinggeli cashmere goat net rate ofcashmere choice traits.4. Moreover, there were found four ideal microsatellite DNA markers, in Xinjiang goat genotype ADof BM1824, in Qinggeli cashmere goat genotype BE of BM1824, genotype CC of BM6506, and genotypeBD of LSCV15, either its fineness or cashmere yield express ideal from the same individual. In terms ofthis finding, it is rare for animals to have good production level in cashmere and wool.