Study On Antidiarrhoeal Activity Of Euphorbia Heyneana Spreng

Euphorbia heyneana Spreng.(Euphorbiaceae Euphorbia Linn.), the same genus as E.humifusa willd. and E. maculate Linn. which were used against diarrhoea in traditionalChinese medicine, has the antidiarrhoeal activity according to Flora of China. However, theacitivity of E. heyneana against diarrhoea has not been studied. The preliminary chemical test,determination of total flavonoids and tannins, antibacterial activity test in vitro andantidiarrhoeal activity test of E. heyneana were undertaken in this study to determine theantidiarrhoeal activity of E. heyneana. The results were as follows:1. Preliminary chemical test and determination of total flavonoids and tannins：Thepreliminary chemical components and content of total flavonoids and tannins of E. heyneanawere determined by test tube method and UV-Vis spectrophotometry, respectively. The resultsshowed that the preliminary chemical components of E. heyneana were similar to E. humifusaand E. maculate, mainly contained flavanoids, flavonoid glycoside, tannins, alkaloids,polysaccharides, glucoside and volatile oils. The content of total flavonnoids and tannins of E.heyneana was22.65mg/g and41.44mg/g, respectively, higher than that of E. humifusa and E.maculate.2. Antibacterial activity test in vitro: The antibacterial activity, minimal inhibitoryconcentration （MIC） and minimal bactericidal concentrations （MBC）of aqueous extract andethyl acetate extract of E. heyneana against Escherichia coli, Salmonella typhimurium,Staphylococcus aureus and Streptococcus agalactiae in vitro were determined by discdiffusion method and broth microdilution method. The results showed that MIC of aqueousextract of E. heyneana against these bacteria was125,62.5,62.5,62.5mg/mL, respectively,and MBC was125,250,62.5,125mg/mL, respectively. MIC of ethyl acetate extract of E.heyneana against these bacteria was1.95,0.49,1.95,0.98mg/mL, respectively, and MBCwas1.95,7.81,7.81,3.91mg/mL, respectively. The ethyl acetate extract of E. heyneana wasthe main principal antibacterial extract and the antibacterial activity of E. heyneana in vitrowas similar to that of E. humifusa and E. maculate.3. Antidiarrhoeal activity test: The antidiarrhoeal activity of aqueous extract and solventextract of E. heyneana was studied by using castor oil-, magnesium sulphate-, E.coli-induced diarrhoea model and intestinal motility test in mice. The results showed that the diarrhoealrates of oil-, magnesium sulphate-, and E.coli-induced diarrhoea model mice treated with E.heyneana at the dose of80mg/10g orally was55.80%,50.90%,48.23%, respectively, and thediarrhea index was0.6148,0.5255,0.5073, respectively. The diarrhoeal rates of oil-andmagnesium sulphate-induced diarrhoea model mice treated with ethyl acetate extraction of E.heyneana at the dose of20mg/10g orally was0,55.32%, respectively, and the diarrhea indexwas0,0.5647, respectively. The diarrhoeal rate of E.coli-induced diarrhoea model micetreated with normal butanol extraction of E. heyneana at the dose of20mg/10g orally was56.32%, and the diarrhea index was0.5632. The propulsion rate of intestinal motility ofmodel mice treated with aqueous extract of E. heyneana at the dose of80mg/10g orally andextraction remains of E. heyneana at the dose of20mg/10g orally was49.55%,44.69%,respectively. The aqueous extract of E. heyneana exhibited treatment effect on exudative,osmotic and bacterial diarrhoea. The mechanisms were suppressing or eliminating entericpathogenic bacteria, suppressing inflammatory exudation and having impacts on the intestinalmobility.