Effect Of Methyl Jasmonate On The Activity Salvia Miltiorrhiza Bunge Enzyme And The Content And Antioxidant Activity Of Phenolic Acid Compounds

Salvia miltiorrhiza Bunge is a perennial herb of Lamiaceae Salvia Linn, which root and rhizome can be medicine. It is the only source for the traditional Chinese medicine S.miltiorrhiza in "Pharmacopoeia of the people’s Republic of China". Zhongjiang county in Sichuan Province is the noted producing areas of S. miltiorrhiza. It is well-known both at home and abroad because of its red color, thick fleshy, and high secondary metabolism output. In this experiment, the S. miltiorrhiza from Zhong jiang County in Sichuan Province is the research object. The experiment discusses with the effect of the methyl jasmonate (MJ) elicitors for the phenolic acids compounds’content of S. miltiorrhiza, the antioxidant activity of phenolic acids, and the activity of SOD, POD, CAT, two kinds of enzyme (phenylalanine ammonialyase and tyrosine aminotransferase activity) which are closely related to the biosynthesis metabolism of phenolic acids. The experiment provides a theoretical basis for the mechanism of elicitor induced S. miltiorrhiza to produce secondary metabolites, which provides technical parameters for making good use of MJ to regulate the secondary metabolite production. The main conclusions are as follows:1. The experiment demonstration the higher the concentration of MJ, the earlier the induction of SOD activity peak appears; MJ of O.Olmol/L-lmol/L induces POD activity peak to appear at3days and3mmol/L at1day. Induction of CAT activity peak is negatively related to the concentration of MJ. The smaller the concentration, the sooner the CAT peak appears, and the higher the concentration, the later the CAT peak appears. MJ elicitor can induce to increase the activity of SOD, POD, and CAT, but the induction time and intensity associate with the concentration of MJ.2. In the experiment of studying the impact of MJ elicitors on the synthesis and accumulation amount of S. miltiorrhiza secondary metabolism, we found that the increased trend of salvia phenolic acid synthesis is in keeping with the activity trends of PAL and TAT. The accumulation of phenolic acid compounds synthesis is accompanied by significantly increased of the PAL’s activity, and the relationship with the change of TAT’s activity was significantly. The above results showed that, MJ can be used as elicitors to stimulate cells’synthesis of phenolic acid compounds, and the induction of phenolic acids in large quantities may be due to activate the activity of TAT PAL of the cells.3. MJ of low concentrations can regulate S. miltiorrhiza to generate phenolic acids compounds strongly. MJ of0.01-0.5mol/L could induce to increase the content of salvia phenolic acid in different extent. After the MJ induction of3days, the best concentration of MJ is0.5mmol/L, the content of phenolic acid of the induced leaflet reached its peak, which is2.33times higher than that of the control group; After the MJ induction of5days, the induction effect of low concentration group (0.01-0.5mmol/L) is significantly higher than that of high concentration (1-3mmol/L). It is mainly due to the high concentration of MJ generate excessive reactive oxygen, and it beyond the scavenging capacity of antioxidant, which cause the serious harm to the cell membrane of S. miltiorrhiza. Induction of MJ can improve the synthesis and accumulation of S miltiorrhiza phenolic acid compounds, but the synthetic accumulation will not increase with elicitor’s concentration increasing all the time.4. After the treatment of MJ, the clearance rate and reducing power of DPPH free radical in different strains S. miltiorrhiza phenolic acid compounds increases, and it is significant difference (P<0.0.1) compared with the control group. This shows that MJ enhances the antioxidant capacity of S. miltiorrhiza in the experiment. In this experiment, the total reducing power four strains of S. miltiorrhiza was the same with the remove effect of DPPH. In the two kinds of antioxidant ability of detection, the four strains are all Tissue culture lobular S.miltiorrhiza leaves>lobular S.miltiorrhiza leaves>grandifoliate S.miltiorrhiza leaves>Tissue culture grandifoliate S.miltiorrhiza leaves. Compared with VC (1mg/mL), we found that the removal rate of ZX-2is higher than VC. The total reducing power of four strains is higher than those of VC. Tissue culture lobular S.miltiorrhiza leaves, lobular S.miltiorrhiza leaves, grandifoliate S.miltiorrhiza leaves, and Tissue culture grandifoliate S.miltiorrhiza leaves are respectively1.32,1.31,1.23, and1.22of VC.