Study On Quality Standard Of Nardostachys.Chinensis Bata1.and Research On Extraction And Inclusion Technology Of Its Volatile Oil

OBJECTIVES:To control the quality of Nardostachys.chinensis Batal., a searies of systemtic research on the quality standerd were studied. And the extraction and inclusion technology were carried out to promoting the stability of its volatile oil, it was a significative thing to the further study on pharmacodynamics.METHODS:The main quality standard research were included in the first part,1.The TLC method was used to preliminary identify the crude drugs.2. The contents of Nardosinonei Chlorogenic Acid and Linarin were determined by HPLC.3.Fingerprints of the materials were also established by HPLC and analysed by Matlab.The extraction and inclusion process we did were as follows,1.We optimized the extraction technology by using the orthogonal design with the evaluation indicator of the yield of oil.2.The orthogonal design was used again to optimize the inclusion technology with the integrated evaluation index, then the TLC and UV methods were used to verify the formation of cyclodextrin inclusion compound.RESULTS:1. The TLC result showed that all the materials were developed except the NO.110517, it was due to the content discretion of the Nardosinone.2. The contents range of volatile oil, Nardosinone, Chlorogenic Acid and Linarin were0.80%～2.95%,0.156%-2.938%,0.071%～0.933%,0.026%～0.253%, respectively, and the methods were accuracy,simple and scientific.3. Common pattern of the fingerprints were established. The fingerprints were measured by similarity analysis, Hierachical clustering analysisand princirpal components analysis, and all the analvsis were tend to coincident.4. The best conditions for its volatilize oil extraction were as follows:the added amount of water was 10times,extract time was10hours,the herbs needn’t to be soaked, and5. the best conditions for its inclusiong were as follows:the process was carried out under60℃for2hours, the ratio of the volatilize oil and the β-CD was1:6. The verifying test showed the the cyclodextrin inclusion compound was formed, and GC-MS analysis of its chemical composition before and after the inclusion did not change a lot.CONCLUSIONS:The content differences of the constituents were exist among the different batches. It maybe due to the difference of harvest, process, store and ecological environment, but obviously, the medicine parts diversity was the main cause. The methods of determination and fingerprint we established were available to the quality control. In the technology part, the results showed that the optimized condition was reasonable, the evaluation index and verify method were scientific. All the work we did provided more foundation to the further study.