Studies On The Preparation And Quality Standard Of Anti-Hyperlipidemia Effective Parts Of Cuscuta Chinensis Herbs

Hyperlipidemia（HLP）is a disease related to lipid metabolism disorders,and if this symptom persists,it can lead to other cardiovascular and cerebrovascular diseases.In clinical practice,western medicine such as statins and bile acid chelators are often used for treatment,but there may be symptoms of treatment resistance and intolerance during the treatment process;researchers have identified traditional Chinese medicine as a new direction for treating hyperlipidemia,Cuscuta chinesis is the dry aerial part of Cuscuta chinesis Lam.（CCL）.It was first recorded in the "Shen Nong Ben Cao Jing" and listed as a top grade.It has the effects of protecting the eyes,delaying aging,and improving fertility,mainly treating black bile or abnormal mucinous diseases,such as cold depression,insomnia,and other symptoms;The previous research by the research group showed that Cuscuta japonica has lipid-lowering activity;Domestic and foreign studies often use it as a medicinal herb in the compound formula for reducing blood lipids.However,there is insufficient depth in the material basis and mechanism of blood lipid lowering effect of the whole plant of Cuscuta chinesis.Therefore,this article takes Cuscuta chinesis as the research object,analyzes its chemical composition,prepares its active parts,and conducts quality standard research to preliminarily speculate and verify its lipid-lowering mechanism.The main content includes:The first part introduces the etiology of hyperlipidemia and its treatment with Western medicine and traditional Chinese medicine;The phytochemistry constituents and pharmacological effects of Cuscuta chinensis were briefly described;The relevant applications of liquid chromatography-mass spectrometry technology in the quality control of traditional Chinese medicine were listed from aspects such as fingerprint and content determination.The second part establishes fingerprint maps of different hosts of Cuscuta chinesis.By using HPLC-Q-TOF-MS technology to analyze the chemical components of Cuscuta chinesis,a total of 67 chemical components were identified,including 3cinnamic acid derivatives;4 alkaloids;8 phenolic acids and their derivatives;14phenylpropanoids;29 flavonoids and 7 other compounds further enriched the material foundation of Cuscuta japonica.The third part uses Hyperin,Isoquercetin,Astragaloside,and Quercetin as indicator components,combined with single factor and orthogonal experiments,to determine the optimal process of Cuscuta chinesis: material to liquid ratio of 1:18,ethanol concentration of 70%,extraction time of 1 hour,extraction frequency of 3times;On this basis,with the help of macroporous adsorption resin test,the optimal purification process was determined by examining the process parameters such as sample concentration and elution ethanol concentration.The sample concentration was 0.4 g · mL^-1 solution,adsorbed at 3 BV · h^-1,and the sample loading and impurity removal water consumption were both 4 BV.Finally,eluted with 70% ethanol at 5 BV,and the eluent was collected.After treatment,the active part of Cuscuta japonica was obtained.The fourth part evaluates the quality of active parts of Cuscuta chinesis through identification,examination and content determination.The fifth part predicts the anti-HLP mechanism of Eridin by means of Chinese traditional medicine system pharmacology,protein analysis and other databases.The initial network of Eridin Eridin’s common targets in the treatment of hyperlipidemia is standardized by CytoNCA plug-ins,and the chemical components of Eridin Eridin are connected,and verified by cell experiments.The results showed that six core targets,including quercetin and apigenin anti-HLP,were selected from Eridopsis Eridis,indicating that Eridopsis eridis may act on HLP through PI3K / Akt / GSK3βsignaling pathway.Cell experiments showed that compared with blank control group,mRNA levels of PI3 K and GSK3β in model group were significantly increased（P <0.01）,while mRNA levels of AKT1 were significantly decreased（P < 0.01）.After the addition of Eridin,the gene（protein）expression levels of PI3 K and GSK3β in the medium and high dose groups decreased with the increase of the dose,with statistical difference（P < 0.01）;the gene（protein）expression levels of AKT1 increased with the increase of the dose of eridin,and the changes in the medium and high dose groups had statistical difference（P < 0.01）.