Conservation Genetics Research On Rhinopithecus Brelichi And Captive Trachypithecus Francoisi

Rhinopithecus brelichi is a unique primate in China and categorized as an endangered species by the IUCN. They mainly distributed in Fanjing Mountain National Natural Reserve. Recent estimates indicate that an overall population is about750individuals. Due to habitat fragmentation and the impact of human activities, the survival situation of Guizhou golden monkeys are not optimistic. In order to protect and manage the R. brelichi, we have carried out research in Mitochondrial DNA and population genetics, the main resualts are as follows:The complete mtDNA of R. brelichi (1,6548bp in length) had13protein-coding genes,22tRNA genes, two rRNA genes, and one uncoding region. Control region sequence was1,087bp without repetitive unit. Protein-coding genes ND6and8tRNA genes located on the light-strand. All the tRNA genes had the typical cloverleaf secondary structures except for tRNASer (AGY) gene.We performed phylogenetic analyses of Colobine monkeys based on complete mitochondrial DNA. The phylogenetic tree by ML showed that Colobine monkeys first diverged African colobines and Asia colobines. Africa colobines constitute Colobus, Procolobus and Piliocolobus. Among the Asia colobines, Semnopithecus diverged first. The relationship of Rhinopithecus and Pygathrix was the closest.We sequenced400bp of the hypervariable I segment from the mitochondrial DNA control region for128individuals. Only one haplotype was identified from these individuals. Compared with other primate species, R. brelichi can be regarded as a species with very low genetic diversity. We need to improve the genetic diversity of R. brelichi and expand the number of population.Mitochondrial DNA control region and microsatellite DNA were used to analysis the genetic diverity, individual origin and relationship of captive Trachypithecus francoisi. For the355bp mtDNA control ragion sequence generated from52individuals,35ariable nucleotide sites defined13haplotypes. It indicated haplotype diversity was0.627and the nucleotide diversity was0.027.11micorsatellite loci amplified well was used in the52samples. The number of alleles was47with an average of4.18. Expected heterozygosity (He) and observed heterozygosity (Ho) were0.559and0.551, respectively. Compaired with other endanger primates, we found that genetic diversity of captive T.francoisi is not low. Anlaysising the phylogenetic relationship of wild T.francois and captive T.francois haplotype, we infer the captive T.francois individuals came from Guanxi.