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Cloning And Analysis Of Resistance-related Genes Against Sclerotinia Sclerotiorum In Arabidopsis Thaliana

Posted on:2011-05-23Degree:MasterType:Thesis
Country:ChinaCandidate:X ZhaoFull Text:PDF
GTID:2230330395955732Subject:Plant pathology
Abstract/Summary:PDF Full Text Request
Oilseed rape is one of the most important economic crops in the world. Sclerotinia sclerotiorum distributes widely and causes destructive disease of many crops including oilseed rape, which seriously restricts the development of oilseed production in China. Currently, no high resistance resources have been found yet, so searching for efficient resistant genes has great significance in controlling strategies against this disease. Results of Quantitive Real-time polymerase chain reaction (qRT-PCR) have proved that JA and ET signaling pathways are involved in interaction between Sclerotinia sclerotiorum and Arabidopsis, but not SA signaling. In this study, four genes induced by Sclerotinia sclerotiorum have been cloned and transformed into Arabidopsis and oilseed rape by Agrobacterium tumefaciens-mediated transformation method. Two Arabidopsis transgenic lines which overexpress AtBOSl possess enhanced resistance to Sclerotinia sclerotiorum, which may provide a clue to develop novel resistance material and a evidence to study molecular mechanisms of interactions between Sclerotinia sclerotiorum and its hosts Arabidopsis and oilseed rape.
Keywords/Search Tags:Arabidopsis thaliana, Sclerotinia sclerotiorum, Resistant-related genes
PDF Full Text Request
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