| Sclerotinia sclerotiorum is a necrotrophic model pathogen that causes great economic losses worldwide.Sclerotium is a dormant structure and plays significantly biological and ecological roles in the life cycle and disease cycle of S.sclerotiorum and other species of sclerotium-forming fungi.mil RNA as one of the non-coding small RNA plays an important regulatory role in the development and pathogenicity of fungi.Sclerotial development is associated with the complicated biological and biochemical regulations.Therefore,mil RNAs associated with sclerotial development of S.sclerotiorum were investigated in this study.A total of 275 mil RNAs were identified with induced expression during sclerotia development by high-throughput sequencing,bioinformatics analysis and analytical biology,in which 51 of mil RNAs were differentially expressed.The putative functions of the predicted target genes corresponsive to the differentially expressed mil RNAs were annotated by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses.The target genes predominantly participated in biological process,cellular components and molecular function in GO analysis as well as metabolism,genetic information and cellular process in KEGG analysis.Furthermore,the expression levels of nine selected mil RNAs coordinated with their corresponsive targets were validated by q RT-PCR assays.Among them,Ssc-mil R-240 may participate in sclerotia development through epigenetic regulation of its target histone acetyltransferase in which indicating that sclerotia development may be related to the regulation of mil RNAs.This study will facilitate the better understanding of the mil RNAs regulation associated with sclerotial development in S.sclerotiorum and other sclerotium-forming fungi.Moreover,such work will provide a novel insight into the molecular regulations of fungal morphogenesis and the potential targets used for the sustainable management of plant diseases caused by S.sclerotiorum. |
PDF Full Text Request