Cloning Of Candidate Genes Response To Oxalic Acid Stress In Arabidopsis Thaliana And Analysis Of Their Resistances To Sclerotinia Sclerotiorum

Arabidopsis thaliana is a model cruciferous plant and can be infected by the pathogen. Therefore, we use Arabidopsis-Sclerotinia sclerotiorum as a model to elucidate their molecular interaction mechanisms, which may provide some clues to breed for rapeseed resisting to Sclerotinia sclerotiorum. This study is based on the differentially expressed gene profile of the model plant Arabidopsis under the stress of oxalic acid, we obtain the genes'encoding informations from the Arabidopsis database TAIR, primarily through the over-expression of the selected gene and get their mutants, then analyze their resistance to Sclerotinia sclerotiorum.Our previous microarray analysis has shown that At1g07890, At1g64160, At2g39350, At2g45570, At3g08970 and At3g20310 were induced when exposed to oxalic acid stress. To study their functions, over-expression vectors are constructed and transformed to the Arabidopsis plants by Agrobacterium-mediated transformation. Plants with over-expression of At1g07890, At2g39350 and At3g20310 were obtained when validated by gene expression level, and plants with over-expression of At1g64160, At2g45570 and At3g08970 were obtained when detected with bar gene. All these mutants showed a significant resistance to Sclerotinia sclerotiorum (p <0.05) in vitro detached leaves. The mutants of At1g07890, At1g64160, At2g39350 and At2g45570 showed increased resistance to Sclerotinia sclerotiorum, while mutants of At3g08970 and At3g20310 had no obvious change of resistance to Sclerotinia sclerotiorum when inoculated with whole plants. Our results suggest that these genes may be good resources of resistance to Sclerotinia sclerotiorum, need further studies.