Cloning Of At1g33730and At4g39230and Functional Analysis Of At1g64160and At2g39350in Arabidopsis Thaliana

Sclerotinia is a worldwide disease caused by necrotropic phytofungus Sclerotinia sclerotiorum with a wide range of hosts. Oxalic acid (OA) is secreted toxin and act as a key pathogenic factor during infection process.In this paper, Atlg64160. Atlg33730, At2g39350and At4g39230were chosen as candidate genes which were highly induced under OA stress. The results are as follows:1. Atlg33730and At4g39230over-expression vectors were constructed, transferred into wild-type Arabidopsis thaliana col-0and basta-resistant transgenic plants were obtained which facilitate the further study of their functions.2. The resistances to Sclerotinia sclerotiorum of Atlg64160and At2g39350overexpression transgenic plants were verified by in vivo inoculation.3. Based on the analysis of promoter elements, signal peptide, hydrophobicity, transmembrane region and the prediction of secondary and tertiary structure of Atlg64160and At2g39350, we found that Atlg64160, which is encoding a disease resistance response protein, may be coordinately regulated and induced by JA and ABA and secret into the extracellular so that enhance the resistance to OA or Sclerotinia sclerotiorum in Atlg64160Over-expression plants; At2g39350encoding the ABC transporter protein, may be affected by multiple regulatory pathways (JA/ET, ABA, or light) induction and involved in plant defense response to Sclerotinia.