Determine And Analyze The Blood Physiological Parameters Of The Giant Panda

Aluropoda melanoleuca is one of the rare endangered species which is the national treasure of China and it has high research and aesthetic appeal value. How to protect the giant panda better, monitoring and evaluating the health status of the giant panda, determining the normal physiological indicators of the giant panda blood, it is very important for monitoring the health of giant panda, disease diagnosis and treatment. This study determined and analyzed the blood physiological parameters for488blood samples from123giant pandas. The main contents as follows:1.Determine and analyze the time period of the stable detection of the giant panda blood physiological parametersIn this study, we took60blood samples with anticoagulant of Giant Panda and saved in room temperature (18℃～25℃) in the China Conservation and Research Center of Giant Panda(CCRCGP) in WoLong of Sichuan. Analyzed the60samples with the MEDONIC CA620automatic blood cell analyzer in two different time period, one time period was within one hour and the other one was interval of24hours. The results as follows:the platelet detecting within1hour is significantly higher than the other one and the significant difference (P<0.05)(respectively205.792and194.874); the platelet volume (respectively6.178and7.356) and white blood cells (respectively9.852and11.369) detecting within1hour is significantly lower than the other one and the significant difference (P<0.05),the other of the physiological parameters were no significant differences (P>0.05). The results show that the better time to detect the giant panda blood physiological indicators should be within1hour; If more than24-hours, platelets, platelet volume, white blood cell maybe changed remarkably (P<0.05).2. Determine and analyze the blood physiological parameters of the giant panda in different states2.1Determine the blood physiological parameters of different gender of giant pandasGot the230female blood samples from78female giant pandas and258male blood samples from45male giant pandas and detected within1hour. The results as follows:the average of physiological parameters were significant difference (P<0.05) between male and female such as red blood cells(male6.453and female6.029), white blood cells(male12.002and female10.137), hemoglobin (male126.433and female120.759). The other physiological parameters were not significant difference (P>0.05). 2.2Determine and analyze the blood physiological parameters in different ages of giant pandasGot20blood samples from the elderly giant panda (age>20years),154samples from adult giant pandas (6-20years old),44samples from sub-adult giant pandas (1.5-5years old),27samples from young giant panda (0-1.5years old) and detected within1hour. The results as follows:the average of physiological parameters are significant difference (P <0.05) between old and adult giant panda such as lymphocytes (elderly19.8713, adult25.5432) and corpuscular hemoglobin concentration (elderly363.3631, adult359.1332).The average of physiological parameters are significant difference (P<0.05) between old and sub-adult giant panda such as red cells (elderly6.1313, sub-adult6.0443) and mean cell hemoglobin (elderly20.3731, sub-adult19.7194). Platelet (elderly205.2135, young227.9198) is significant difference (P<0.05) between old and young giant panda (elderly205.2135, young227.9198). White blood cells (adult13.2061, young9.63945) is significant difference (P<0.05) between adult and young giant panda. Mean Corpuscular Volume (sub-adult53.3743, young51.7312) is significant difference (P<0.05) between sub-adult and young giant panda. The other physiological parameters were not significant difference (P>0.05).2.3Determine and analyze the blood physiological parameters of estrus and non-estrus giant pandaGot56blood samples from estrus giant pandas and87blood samples from non-estrus giant pandas and detected within1hour. The results as follows:The average of physiological parameters are significant difference (P<0.05) between estrus and non-estrus giant pandas such as platelet (estrus207.8908, non-estrus247.945), white blood cells (estrus14.0891, non-estrus10.8509) and thrombocytocrit (estrus0.1160, the estrus0.1431). The other physiological parameters were not significant difference (P>0.05).2.4Determine and analyze the blood physiological parameters of giant panda in different seasonsGot185,187,42and46blood samples respectively in spring, summer, autumn and winter and detected within1hour. The results as follows:The average of physiological parameters are significant difference (P<0.05) between spring and summer such as Mean corpuscular hemoglobin (spring373.6848, summer357.0573) and neutrophils (spring59.7935, summer66.7919). The average of physiological parameters are significant difference (P<0.05) between spring and autumn such as White blood cell(spring10.8821, autumn9.6244), the absolute number of lymphocytes (spring2.864, autumn1.8829). Red blood cell distribution width number (spring41.4334, winter37.3761) is significant difference (P<0.05) between spring and winter. The average of physiological parameters are significant difference (P<0.05) between summer and autumn such as mean corpuscular volume (summer54.7152,autumn58.4780) and hematokrit (summer34.0478,autumn35.4878). Cell absolute number (autumn6.5876, winter8.3741) is significant difference (P <0.05) between autumn and winter.The other physiological parameters were not significant difference (P>0.05).2.5Determine and analyze the blood physiological parameters of anesthesia and non-anesthesia giant panda.Got80blood samples from anesthesia giant pandas and120blood samples from non-anesthesia giant pandas and detected within1hour. The results as follows:The average of physiological parameters are significant difference (P<0.05) between anesthesia and non-anesthesia such as platelet (anesthetic207.8909, non-anesthesia237.9455), white blood cells (anesthesia12.0455, non-anesthesia10.8509), corpuscular hemoglobin concentration (the anesthesia398.1273, non-anesthesia364.3727). The other physiological parameters were not significant difference (P>0.05).3. The reference range of blood physiological parameters of panda Through analyzing the stability and different states of the blood physiological indicators, the reference range of giant panda can be summarized as follows:red blood cells4.12～8.64; platelet89.26～386.12; leukocyte5.78～13.85; hemoglobin85.58～166.60; lymphocyte absolute number0.10～5.63; average hemoglobin concentration17.48～22.52; the absolute number of neutrophils:0.50～12.66. In this study, we determined20blood physiological parameters in different genders and age ranges of the giant pandas through analyzing488blood samples which brought valuable scientific data for many giant panda veterinary workers and had important clinical significance.