Study On Multi-channel Microchip Of Capillary Electrophoresis And Detection System

In order to meet the substantive demands of synchronous detection and assay in Human post-genomic project, the research of epidemic disease and the composition analysis of medicament, the thesis presents a multi-channel scanning examination system of Capillary Electrophoresis based on optical fiber characteristic of little volume, high sensitivity and low power. The system substituted fiber for complex and large-volume con-focal optical path in traditional LIF detection system. The APD was used as fluorescent detecting unit, the brace moved by the DC machine completed the multi-channel scan.STM32F103 SOC was adopted as the control unit of embedded system of data processing and controlling which completed functions as follows: the acquirement of high-speed fluorescent signal, , the multi-channel recognition and the communication with upper computer. It reduced complexity of circuit design effectively and enhanced controlling system's ratio of performance to price. APD was used as photoelectric converting device in optical-to-electric signal detection, implementing small volume and high-gain measurement of fluorescent signal.The software of detection system was consist of embedded software and data processing software in which theμCOS-Ⅱimplemented embedded software for terrace and brought a great convenience for establishment of parallel multi-task and inter-task communication. It also decreased software developing complexity of system's functional perfection when it was upgraded. Upper-computer software accomplished functions as signal collection, preservation of data files and graphic display. To accomplish data collection and processing in PC, multithreading mode was utilized, reducing CPU's occupancy factor by data processing software effectively.The device designed in this thesis can accomplish multitasks preferably, for instance: collection of fluorescent signal in multi-channel capillary electrophoresis chip, channel identification, curve drawing of fluorescent signal. The device achieves expectation effect basically with its small volume and easy of use.