Anti-osteoporosis Effects Of The Standard Extract Of Pueraria Lobata

Object: The standard extract of Pueraria Lobata (SEP) was extracted from the traditionalChinese medicine Pueraria Lobata. In this paper, we examined the Anti-osteoporosispharmacology effect of SEP.Methods and results:HPLC was introduced to evaluate the characteristic composition of the puerarin. This methodwas reliable and repeatable. Based on the character ratio of the puerarin, by adjusting theextracting rate of the puerarin, the producing rate of extract, and expansion rate of the bloodvessel in vitro of the rabbit by SEP, the extracting process was optimized. It is found that theconcentration of the solvent, temperature, extracting time and the quantity of the solvent arethe main factors for the process. The optimized condition was the double extractingprocedure in 75% ethanol at 80℃. It costed 60 mins in 12 times solvents in first procedureand 60 mins in 10 times solvents in the second one.By evaluating the LC results of the SEP, it can be found that the peak areas of the No.2,No.3 and No.7 were various, which apparently should be related to the expansion rate of theblood vessel in vitro.The biological effect of SEP was evaluated by adopting ovariotomy rats model simulatingosteoporosis model, and characterized from thighbone measurement, bone density, bloodbiochemistry, emiction biochemistry, bone morphology and womb pathology. The ovariotomy causes rats' weight, serum ALP, Strap, bone calcium element, emiction calcium element, emiction hydroxide praline increased; bone calcium content, bone density and rigidity, thighbone wet weight,,dry weight and ash weight remarkably reduced; and the bone anti-bend intensity, the maximum bend strength decreased, the bone girder thinner, or even broken. This indicates that ovariotomy causes rats lost bone calcium and got osteoporosis. One month after operation, SEP was provided subsequently for 12 weeks to different samples (medicament quantity 2.5, 1.25, 0.625 g/kg; model group; fake operational group and masculine medicament estrogen group). It is found that SEP can suppress the weight increase caused by ovariotomy, 13.5%, 13.1% and 9.3% separately; improve the thighbone metrology standard of the OVX rats (the bone calcium content 26.2%, 20.1% and 15.7% separately); increase the thighbone density and rigidity, the thighbone wet weight,,dry weight and ash weight; improve the blood biochemistry and immunology character: increasing blood calcium, blood phosphorus, decreasing ALP, Strap, increasing serum estradiol level, reducing bone calcium content; improve the emiction biochemistry characteristic: decreasing emiction calcium, emiction hydroxide proline ejection amounts; improve the OVX rats thighbone biomechanics characteristic: increasing the anti-band intensity and maximum bend strength. Moreover, the stimulating proliferation effects to the womb of SEP are less than estrogen.Conclusion: In summary,, SEP is proved to have remedial effects on osteoporosis of rats caused by ovariotomy.