Differential Expression Profiles Of MicroRNAs Between Sarco-osteoporosis And Osteoporosis

Objective:To explore the differentially expressed miRNA profiles between sarco-osteoporosis and osteoporosis.Methods:The samples of muscle tissues from patients with sarco-osteoporosis and osteoporosis were collected,Illumina high throughput sequencing technique was used to analyze and identify the differentially expressed miRNAs between the two groups.The results were confirmed by quantitative real-time PCR（qRT-PCR）.Four differentially expressed miRNAs（hsa-miR-381-3p,hsa-miR-29b-3p,hsa-miR-433-3p,hsa-miR-182-5p）that have been validated were predicted,and the target genes were analyzed by gene ontology（GO）enrichment analysis,Kyoto gene and genome Encyclopedia（KEGG）signaling pathway analysis.Results:Compared with OP group,212 differentially expressed miRNAs were identified in SO group,among them,34 were significantly dysregulated（︱log₂（fold-change）︱≥2 and P<0.05）,including 22 up-regulated miRNAs and 12down-regulated ones.The qRT-PCR results were consistent with miRNA high throughput sequence results.The GO analysis showed that target genes were enriched in immune response,receptor-protein binding,metabolism and other biological processes.KEGG pathway analysis showed that the target genes of differentially expressed miRNAs were significantly enriched in osteoclast differentiation,cytokine receptor interaction,NF-κB signaling pathway,and Notch signaling pathway.Conclusions:Our study found 34 differentially expressed miRNAs in patients with sarco-osteoporosis and osteoporosis.Among these miRNAs,hsa-miR-381-3p,hsa-miR-29b-3p,hsa-miR-433-3p and hsa-miR-182-5p provide valuable clues for further study of the pathophysiological process of bone loss in patients with sarcopenia.However,in order to provide a basis for the prevention and treatment of musculoskeletal diseases in clinic,we need to dig and validate the screened miRNAs and their target genes more comprehensively and thoroughly in the future.