| ObjectiveRecently people investigate Bile Duct Stones of cause and discover that inordinate stomach-intestines duct and bile duct power play an important role in Bile Duct Stones. stomach-intestines hormone adjust stomach-intestines duct and bile duct motion .The change of a or several sort of stomach-intestines hormone cause dysfunction of stomach-intestines duct and bile duct function and change of bile component.Bile mud gradually appear and stone form. Some Chinese traditional medicine with adjusting stomach-intestines hormone are discovered along with development of modern times Chinese herbal medicine.According to elicitation, let Primary Bile Duct Stones Patients take Jinhuangyidankeli in order to measure the content of MTL SS and Sp in plasma with radioimmunoassay to Probe into the mechanism that Jinhuangyidankeli prevent Primary Bile Duct Stones Patients who were operated on the bile duct stones from reforming stoneMaterials and Method1. Materials1.1 Main instrument and apparatus: SHINKO一SH一210R Electronic analytic balance; GC-2016r radio-immunity counter;LDR4—8.4Cclinical centrifuge;SANYO-70℃ultra cold freezer;1.2 Main reagents: aprotinin (40,000kiu/ml,2ml/bottle); 7.5% EDTA一2Na(2ml/bottle); radio-immunity kit for motilin; radio-immunity kit for somatostatin; radio-immunity kit for substance P.2.Method2.1 Subjects The samples came from 66 primary or secondum gallstone patients admission to Kailuan Hospital in the period from May 2008 to May 2009. The normal control group included 10 healthy staff and family members of the patients. The above objects had not taken some medicine which can influence the power of gastrointestinal tract and biliary tract.The suitable operation means determined by the location of the gallstone.The gallstone had been taken out by the choledochoscope in operations of all patients. The excluding standard included the patients who had cholecystolithiasis, acute obstruction suppurative cholangitis,other gastrointestinal tract disease, diabetes, fatty liver, hepatic cirrhosis ,serum hepatitis, hyperlipemia, no tolerance to the drugs, simple biliary tract–bowel connection operation. The standard also included pregnancy or preparing pregnancy women and lactation women. All the persons were randomly divided into the treatment group, the control group and the healthy group. Age and gender were seen from the following table1.2.2 The ways taking medicineAll the patients had been accepted anti-inflammatory and protecting liver treatment before or after operation. Any medicine can influence the power of gastrointestinal tract and biliary tract were forbidden. The test began when the patients can take semiliquid diet 3 days after operation, and they have no abdominal pain, febrile, constipation, and blood clot and duodenum juice cannot be seen in the drainage pack. All the persons had normal food and drink.The treatment group: taking Jinhuangyidankeli every day,1 bag 1 time ,2 times 1 day. 20g /bag.The control group: having normal food and drink, without taking any Jinhuangyidankeli.The normal(healthy) groups :having normal food and drink.2.3 Samples collectionThe treatment group: Taking fasting venous blood 4 times by precooling injector. (1) 3 ml fasting venous blood before operation;(2) 3 ml fasting venous blood after taking 3 days jinhuangyidankeli after operation;(3) 3 ml fasting venous blood after taking 10 days jinhuangyidanli after operation;(4) 3 ml fasting venous blood after aking 45 days jinhuangyidankeli after operation.The control group: Taking fasting venous blood 4 times by precooling injector.(1) 3 ml fasting venous blood before operation;(2) 3 ml fasting venous blood after 3 days meals after operation;(3) 3 ml fasting venous blood after 10 days meals after operation;(4) 3 ml fasting venous blood after 45 days meals after operation.The normal(heathy) group: Taking fasting venous blood 1 time by precooling injector.Put the venous blood into the precooling test tube which had EDTA-2Na and Inhibitory skin enzyme. Centrifuged it 15minutes at 4℃(3000 turns/minute).Put the samples after Centrifuging into 3 test tubes.Keep them at -70℃.2.4 Radio-immunity test of motilin, somatostatin and substance P2.5 Statistics analysisThe data will be processed by spss 13.0 software. The data may be in line with normal distribution. To compare the mean of the fasting plasma gut hormone levels of the treatment group and the control group, we use repeating measurement analysis of variance. To compare the mean of the first and the fourth fasting plasma gut hormone levels in the treatment group and the control group with the mean of the gut hormone levels of the normal(healthy) group, we use fully random analysis of variance. The data demonstrate by x±S. The comparisons have statistical significance if p<0.05.Results1. Plasma motilin levels (1)There is no obvious difference and statistical significance in the first fasting plasma motilin levels between the treatment group and the control group(P>0.05).The same happens in the fourth fasting plasma motilin levels between the two groups(P>0.05).(2)There is obvious difference and statistical significance between the fasting plasma motilin levels of the normal group and the first fasting plasma motilin levels of other two groups, the control and treatment groups(P<0.05) .It indicates that the normal fasting plasma motilin levels are higher than the fasting plasma motilin levels in the treatment group and control group before operation.(3)There is no obvious difference and statistical significance between the fasting plasma motilin levels of the normal group and the fourth fasting plasma motilin levels of other two groups,the contol and treatment groups(P>0.05) .Among the three groups ,the plasma motilin levels of the normal group is the highest,the levels of the control group is the lowest. 2. Plasma somatostatin levels.(1)There is no obvious difference and statistical significance in the plasma somatostatin levels between the treatment and control groups(P>0.05).(2)There is obvious difference and statistical significance in the third and the fourth fasting plasma somatostatin levels between the treatment group and the control group( P<0.05).It indicates that the fasting plasma somatostatin levels of the third and the fourth in the treatment group is lower than the levels of the control group.(3)There is obvious difference and statistical significance in the first fasting plasma somatostatin levels of the treatment group between the fasting plasma somatostatin levels of the normal group(P<0.05).The same happens in the first fasting plasma somatostatin levels of the control group between the fasting plasma somatostatin levels of the normal group(P<0.05).It indicates that the fasting plasma somatostatin levels of the treatment and control group are all higher than the plasma somatostatin levels of the normal group.(4)There is no obvious difference and statistical significance in the fourth plasma somatostatin levels of the treatment group between the fasting plasma somatostatin levels of the normal group(P>0.05). There is obvious difference and statistical significance in the fourth plasma somatostatin levels of the control group between the fasting plasma somatostatin levels of the normal group.It indicates that the fasting plasma somatostatin levels is similar to the levels of the normal group. The fasting plasma somatostatin levels of the control group is higher than the levels of the normal group.3. Plasma substance P levels(1) There is no obvious difference and statistical significance in the first fasting plasma substance P levels between the treatment group and control group(P>0.05).(2)There is obvious difference and statistical significance in the fourth fasting plasma substance P levels between the treatment group and control group(P<0.05).Clearly, the fourth fasting plasma substance P levels of the treatment group is higher than the levels of the control group.(3)There is obvious difference and statistical significance in the first fasting plasma substance P levels between the treatment group and normal group, or between the control group and normal group. (P<0.05).Clearly, the fasting plasma substance P levels of the treatment group and control group are all lower than the levels of the normal group.(4) There is obvious difference and statistical significance in the fourth fasting plasma substance P levels between the treatment group and normal group, or between the control group and normal group(P<0.05).Clearly, the fasting plasma substance P levels of the treatment group and control group are all lower than the normal group, but the levels of the treatment group are near to the levels of the normal group.Conclusion1.The fasting plasma motilin and substance P levels of the healthy persons are all significantly higher than the above two substance levels of the primary gallstone patients. Compared with the primary gallstone patients, the plasma somatostatin levels of the healthy persons are significantly lower.2.With regard to the primary gallstone patients after operation, the motilin, somatostatin and substance P levels of the naturally recovery group and the treatment group all have a changing process. After one and a half month, the somatostatin and substance P levels in the patients taking jinhuangyidan drug can achieve the normal levels of the healthy persons. For motilin, comparing in every two groups, it's levels have no statistical significance, but it's levels of the treatment group is the nearest to the levels of the healthy persons. From this, it can infer that taking jinhuangyidan drugs can make the above three plasma substance levels of the patients normal .It indicates that jinhuangyidan drugs can prevent the primary gallstone patients from recurring. |
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