Influence Of Tongfu Decoction On SP-A In PS In Lung And Intestine Tissue Of Rats With Acute Lung Injury

Objective:To observe the effect on SP-A in PS in ALI rat lung and intestine, and to discuss the mechanism of Tongfu Decoction anti-ALI.Method:180 Wistar rats were divided into 6 groups randomly:the normal control group, the model group, hexadecadrol group, Chinese herbal medicine low-dose group, medium-dose group, and high-dose group,30 rats each group. Giving respectively the Chinese herbal medicine intragastric administration and Dexamethasone intraperitoneal injection, copying ALI model by intraperitoneal injection LPS half an hour later, and executing rats in 1,2,6 hour after treatment respectively,10 rats each time in each group. We observed lung and intestine histomorphology changes, detected SP-A in PS contents in lung and intestine by ELISA method synchronously.Result:1.Lung and intestine in the model group showed dark red, turgent, bleeding by naked eye, but other treatment groups showed lighter than it.2.Lung and intestine in the model group showed inflammatory cell infiltrating, edema, hyperemia, disorganization in light microscope, but other treatment groups showed lighter.3. SP-A in PS contents increased as time extending in first 6 hours in lung and intestine of the model group.4.Lung and intestine in ALI:SP-A in PS contents in the model group were lower than the normal control group (p<0.01); SP-A in PS contents in hexadecadrol group and Chinese herbal medicine groups were higher than the model group (p<0.01); SP-A in PS contents in Chinese herbal medicine low-dose group were lower than hexadecadrol group (p<0.05); SP-A in PS contents in Chinese herbal medicine medium-dose group,high-dose group and hexadecadrol group had no statistical significance (p>0.05); SP-A in PS contents in Chinese herbal medicine low-dose group were lower than Chinese herbal medicine medium-dose group and Chinese herbal medicine high-dose group (p<0.05); SP-A in PS contents in Chinese herbal medicine high-dose group and Chinese herbal medicine medium-dose group had no statistical significance (p>0.05)Conclusions:Tongfu Decoction could increase SP-A in PS contents in lung and intestine of ALI rats, medium-dose group and high-dose group were better.