The Influence Of Tongfu Decoction On VIP In Lung And Intestine Of Acute Lungn Injury Rats

Objective:To observe the effect on VIP in ALI rat model lung and intestine, and to approach the mechanism of Tongfu Decoction anti-ALI.Method:180 Wistar rats were divided into 6 groups randomly: the normal control group, the model group, hexadecadrol group, Chinese herbal medicine low-dose group, medium-dose group, and high-dose group, 30 rats each group. Copying ALI model by caudal vein injecting LPS, giving respectively the Chinese herbal medicine intragastric administration and Dexamethasone intraperitoneal injection half an hour later, and executing rats in 1,2,6 hour after treatment respectively, 10 rats each time in each group. We observed lung and intestine histomorphology changes, detected VIP contene in lung and intestine by ELISA method synchronously.Result:1 Lung and intestine tissue in the model group showed dark red, turgent, bleeding by naked eye, but other treatment groups showed lighter than it.2 Lung and intestine tissue in the model group showed inflammatory cell infiltrate, edema, hyperemia, disorganization in light microscope, but other treatment groups showed lighter.3 Model VIP levels in lung tissue reached a peak after 1 hour, VIP content in intestinal tissue was not detected peak.4 Lung and intestine tissue in ALI: VIP contents in the model group were higher than the normal control group (P< 0.05);contents in hexadecadrol group and Chinese herbal medicine groups were lower than the model group (P< 0.05);contents in Chinese herbal medicine low-dose group were higher than hexadecadrol group (P < 0.05);contents in Chinese herbal medicine medium-dose group and high-dose group and dexamethasone group had no statistical significance (p > 0.05);contents in Chinese herbal medicine medium-dose group and high-dose group showed lower than low-dose group (P< 0.05);Chinese herbal medicine medium-dose group and high-dose group had no statistical significance (p> 0.05).Conclusions:1 Tongfu Decoction could decrease VIP in lung tissue of ALI rats model.2 Tongfu Decoction could decrease VIP in intestine tissue of ALI rats model.