The Influence Of Tongfu Decoction On Sp In Lung And Intestine Of Acute Lungn Injury Rats

Objective:To observe the effect on substance P(SP) in lung and intestine tissue of ALI rat model treated by Tongfu decoction, and explore the mechanisms of Tongfu decoction anti-ALI, so to provide experimental base on the lung and the large intestine being interior-exteriorly related theory.Methods:180 Wistar rats were divided into 6 groups randomly:the normal control group, the model group, hexadecadrol group, Chinese herbal medicine low-dose group, medium-dose group, and high-dose group,30 rats each group. Copying ALI model by caudal vein injecting LPS, giving respectively the Chinese herbal medicine intragastric administration and Dexamethasone intraperitoneal injection half an hour later, and executing rats in 1,2,6 hour after treatment respectively,10 rats each time in each group. We observed lung and intestine histomorphology changes, detected SP content in lung and intestine by ELISA method synchronously.Result:1 Lung and intestine tissue in the model group showed dark red, turgent, bleeding by naked eye, but other treatment groups showed lighter than it.2 Lung and intestine tissue in the model group showed inflammatory cell infiltrating, edema, hyperemia, disorganization in light microscope, but other treatment groups showed lighter.3 SP content increased in lung tissue of the model group, while it decreased in intestine tissue as time extending in first 6 hours.4 Compared with normal control group, There was higher SP expression in lung tissue and lower SP expression in intestine tissue in model group (P<0.05). SP decreased in lung tissue and increased in intestine tissue significantly in treatment groups Compared with model group (P<0.05). Compared with dexamethasone group,SP level is higher in lung tissue and lower in intestine tissue in low-dose group (P<0.05).In high-dose group,medium-dose group and dexamethasone group,SP level in lung and intestine tissue was similar, which showed no significant deviation (P>0.05). SP in low-dose group is higher in lung tissue and lower in intestine than high-dose and medium-dose group (P<0.05). In high-dose group,medium-dose group,SP level in lung and intestine tissue was similar, which showed no significant deviation (P>0.05).Conclusion:1 Tongfu Decoction could decrease SP in lung tissue of ALI rats model, the high-dose and medium-dose group showed better effects.2 Tongfu Decoction could increase SP in intestine tissue of ALI rats model, the high-dose and medium-dose group showed better effects.