The Influence Of Tongfu Decoction On Tgf-β1 In Lung And Intestine Of Acute Lungn Injury Rats

Objective: To observe the effect on TGF-β1 in ALI rat lung and intestine, and to approach the mechanism of Tongfu Decoction anti-ALI, so to provide experimental base on the lung and the large intestine being interior-exteriorly related theory.Method: 180 Wistar rats were divided into 6 groups randomly: the normal control group, the model group, hexadecadrol group, Chinese herbal medicine low-dose group, medium-dose group, and high-dose group, 30 rats each group. Copying ALI model by caudal vein injecting LPS, giving respectively the Chinese herbal medicine intragastric administration and Dexamethasone intraperitoneal injection half an hour later, and executing rats in 1,2,6 hour after treatment respectively, 10 rats each time in each group. We observed lung and intestine histomorphology changes, detected TGF-β1 contene in lung and intestine by ELISA method synchronously.Result:1.Lung and intestine in the model group showed dark red, turgent, bleeding by naked eye, but other treatment groups showed lighter than it.2.Lung and intestine in the model group showed inflammatory cell infiltrating, edema, hyperemia, disorganization in light microscope, but other treatment groups showed lighter.3.TGF-β1 content increased as time extending in first 6 hours in lung and intestine of the model group.4.Lung and intestine in ALI: TGF-β1 contents in the model group were higher than the normal control group(p<0.05);contents in hexadecadrol group and Chinese herbal medicine groups were lower than the model group(p<0.05);contents in Chinese herbal medicine low-dose group were higher than hexadecadrol group(p<0.05);Chinese herbal medicine medium-dose group,high-dose group and hexadecadrol group had no statistical significance (p>0.05);contents in Chinese herbal medicine medium-dose group and high-dose group showed lower than low-dose group(p<0.05);Chinese herbal medicine medium-dose group and high-dose group had no statistical significance (p>0.05).Conclusions:1.Tongfu Decoction could decrease TGF-β1 in lung of ALI rats, medium-dose and high-dose group were better.2.Tongfu Decoction could decrease TGF-β1 in intestine of ALI rats. medium-dose and high-dose group were better.