| Background and objectivesEsophageal carcinoma is a common carcinoma of upper digestive system which severely harms to people s health. China is a esophageal carcinoma incidence and one of a high mortality country of the world. Among them 90% are squamous cell cacrinomas. There are around 150 thousand pantients dead of this disease annually in China. The epidemiological data show that eating habits, living environment, the genetic factors, gender, age, occupation, race and geography have a close relation-ship with esophageal carcinoma. With the development of molecular biology, the study found that more gene alterations were involved in the esophageal cancer which is the result of oncogene activation and anti-oncogene inactivation.Protein tyrosine kinase (PTKS) is a group of poenzyme which can catalyze protein tyrosine residues phosphorylated and participates in many signal transductions. But it plays an important role in controlling cell differentiation, proliferation and diffusion. At present, the study has found that more than 90 kinds of with PTKS have oncogene functions. However, spleen tyrosine kinase is currently the only discovery has tumor-suppressor role of PTKS. Many recent studies show that syk express defects may have the relationship with a variety of tumor formation and metastasis.Ki-67 is a nuclear antigen residing in proliferating cells and has two peptide chains formed, it exists in the whole cell cycle and its expression has relation with different cell cycle. Without expression in GO stage, appearing from G1 mid to late. Gradually increasing from S stage to G2 period and peaking in M1 period. Immediately missing antigen decided to cluster or degradating after M period. Currently, Ki-67 is a most mark of reflecting cell proliferation level and also is one of studying hot spot.The reports of study on SYK and ki67 gene together in esophageal cancer has not been Found in our country. Their effect on the esophageal cancer occurrence is not very clear. The study is ready to detect the expression of tumor suppressor gene SYK and cell cycle depended on kinase inhibitor ki67 in esophageal cancer through RT-PCR method, analyse the relation-ship bewteen them and the tumor grade or phase, and exame the correlation of the expression of these two mRNA in esophageal cancer.The result may be presumed upon to provide experimental evidence on mechanism of occurrence and development of the esophageal cancer, and the treatment on molecular level.Materials and Methods1.48 cancer tissues and 48 adjacent normal tissues are obtained from the patients with esophageal carcinoma who underwent resection in our hospital from Jun. to Dec. in 2010. All cases with complete clinical data and pathologic diagnosis are not cured with chemotherpy and radiotherapy.2. RT-PCR is employed to determinate the gene expression of SYK, KI67 in the 48 cases cancer tissue and 48 cases adjacent normal tissues, while analysing the relationship of gene expression of SYK, KI67 and clinical pathological feature in cancer tissues.3. SPSS 10.0 software application is used for all analysis, and statistical significance is defined as a value less than 0.05.Resultsl.The mean expression of SYK mRNA in cancer tissues is significantly lower than that in nomal esophageal tissues(0.859±0.105 vs 1.165±0.154, P<0.05).2.The mean expression of KI67 mRNA in cancer tissues is significantly higher than that in nomal esophageal tissues(1.007±0.111 vs 0.432±0.067, P<0.05).3. The mean expression of Syk mRNA in the positive lymph node metastasis cancer tissue and the negative lymph node metastasis cancer tissue has significantly diffenent. (0.803±0.078,0.944±0.083, P<0.05) The mean expression of Syk mRNA in the well-differentiated and moderately differentiated and poorly differentiated cancer tissue has significantly diffenent. (0.987±0.057,0.852±0.066,0.761±0.082, P<0.05); Increased abundance of Syk mRNA is relative to esophageal cancer TNM stage. The mean expression of Syk mRNA is diffenent inⅠ,Ⅱ,Ⅲ. (0.970±0.075,0.841±0.063,0.745±0.071, P<0.05). But their relations to the category of tumor, sex are not significant(P<0.01).4. The mean expression of KI67 mRNA in the positive lymph node metastasis cancer tissue and the negative lymph node metastasis cancer tissue is not significantly. (1.021±0.022,0.986±0.021, P>0.05). The mean expression of KI67 mRNA in the well-differentiated and moderately differentiated and poorly differentiated cancer tissue has significantly. (0.890±0.057,0.989±0.061 1.140±0.078, P<0.05); Increased abundance of KI67 mRNA is not correlated with esophageal cancer TNM stage. The mean expression of K167 mRNA is not statistically significant inⅠ,Ⅱ,Ⅲ. (0.968±0.126,1.011±0.070,1.053±0.157,P >0.05). But their relations to the category of tumor, sex are not significant(P> 0.05).5.There is a significantly negative correlation between SYK mRNA and KI67mRNA((r=-0.218, P<0.05).Conclusion1. The positive rates of SyK mRNA expression in nomral esophageal tissues were higher than in esophageal cancer, and are correlated with pathological grade, the clinical stage and lymph nodes metastasis of esophageal cancer. It can reflect the biological feature of esophageal cancer, then, it should be helpful to evaluate the identification of pathological grade and the clinical stage of esophageal cancer.2. The positive expression of Ki67 mRNA expression in nomral esophageal tissues was lower than in esophageal cancer, and was irrelevant to pathological grade, the clinical stage and lymph nodes metastasis of esophageal cancer except differentiation. It can reflect the biological feature of esophageal cancer, then, it should be helpful to evaluate the identification of pathological grade and the clinical stage of esophageal cancer.3. There is lower expression of SyK mRNA and highter expression of KI67 mRNA in esophageal cancer tissues, and a significant negative correlation between them.4. SyK and Ki67 may be important targets for therapy in patients with esophageal cancer. |
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