| Objective: The abnormal expressions of P53, NM23 and Ki67 genes have been shown to be implicated in the occurrence and development in many kinds of human cancers including esophageal cancers.However,there is less research about the the relation between the co-detection of the three proteins and esophageal cancer.Up to now,no specific biomarker has been reported to be sensitive and specific for esophageal cancer detection.But we could improve the detection rate of tumor by comprehensive analysis of a variety of cancer gene and complementary effect.The co-detection of these multi-cancer genes expression profiles,therefore,might be helpful for the diagnosis and prognostic evaluation for esophageal cancer.In the present study,P53, NM23 and Ki67 genes expression have been co-detected and their associations with the clinicopathological characteristics of the disease have been investigated with the aim to elucidate the roles of these genes in esophageal cancer occurrence and development.This method can possible provide science information in esophageal cancer diagnose,treatment and the prognostic assessment.Methods: 120 patients of esophageal cancer postoperative tissue samples were gathered from the Department of Pathology of the Second People Hospital of Yancheng and the First People Hospital of Yancheng between Januaryand December in 2012.Immunohistochemical stains were used to investigate the expression of P53, NM23 and Ki67 in 120 cases of esohageal cancer and signed respectively.The positive cell was dyed maize.Basis for displayed,taked down the number of positive cell.The expression of P53 was 1 suit;The expression of Ki67 was 2 suit;The expression of NM23 was 3 suit;We divided the 120 cases into age,gender,tumor length,lymph node metastasis or not,infiltration depth,tumor location and Clinical stage.Study all above relations.then datas were analyzed by SPSS17.0 software pack.A Chi-square test and and the Spearman correlation was used in this test.P<0.05 was considered as statistical significance. Results: P53 and Ki-67 pitch in nucleolus, NM23 pitches in cytoplasm. In the 120 cases of esophageal paraffin-embeded samples,the P53 positive group was 80 cases,the negative group was 40 cases; the NM23 positive group was 84 cases, the negative group was 36 cases; the Ki67 positive group was 66 cases,the negative group was 54 cases In the study of the esophageal carcinomas,the expression of P53 and NM23,Ki67 with age,length of tumor,tumor site were no significant correlation(P>0.05).The expression of P53 in tumor T stage(P < 0.001)and Clinical stage(P < 0.05)were significantly related.With the increasing degree of infiltration depth in T stage and Clinical stage later,P53 has higher expression.There were no significant differences in gender,degree of differentiation,lymph node metastasis(P>0.05). The expression of NM23 in T stage, Clinical stage and the degree of differentiation were no significant correlation(P>0.05). It was significant with gender(P<0.05) and lymph node metastasis(P<0.05).The expression of NM23 was higher in the case of male patient without lymph node metastasis.While the expression of Ki67 with gender, T stage, Clinical stage have no significant differencs(P>0.05).It was not significant with the degree of differentiation and lymph node metastasis(P<0.05).The expression of Ki67 was higher in the lower differentiation and lymph node metastasis case. Univariate analysis of prognosis shows :length of tumor(χ2=22.548, P<0.001), lymph node metastasis(χ2=17.541, P < 0.05),T stage(χ2=56.903, P < 0.05),Clinical stage(χ2=68.398, P < 0.05)and P53 attribute(χ2=16.166, P < 0.05)have a significant influence on survival;and gender(χ2=3.195, P>0.05), age(χ2=2.126, P>0.05),degree of differentiation(χ2=3.852, P>0.05)and tumor site(χ2=1.866, P>0.05)have no effect on survival.Cox multivariate analysis of prognosis shows:the risk factors affecting overall survival are length of tumor(B=0.754, P<0.05), lymph node metastasis(B=0.530, P<0.05),T stage(B=1.382, P<0.05).Conclusion: 1、P53 was showed significant difference in T stage and Clinicalstage;In the cases with T stage and Clinical stage increased,the positive expression rate of P53 was higher,This showed the expression of P53 associated with the biological behavior,invasion and metastasis of esophageal carcinoma. NM23 is a tumor suppressor gene.It was significant difference in gender and lymph node metastasis or not. There was higher expression in the cases with male and no lymph node metastasis.There was lower expression of NM23 in the cases with lymph node metastasis. Ki67 was showed significant difference in esophageal carcinoma with differentiated degree,Lymph node metastasis or not. The lower differentiation the higher positive expression rate. Lymph node metastasis showed the higher expression rate. 2、Analysis of single factor affecting the prognosis showed that length of tumor,lymph node metastasis,T stage,Clinical stage,P53 attribute has obvious influence on survival,and gende,age,degree of differentiation,tumor site had no effect on the prognosis and survival.3、Cox multivariate analysis of prognosis showed that the influential risk factors for survival were length of tumor,lymph node metastasis,T stage,Clinical stage,P53 attribute. |
PDF Full Text Request