Study Of Laparoscopic Abomasal Fistula Surgery In Sheep

Compared with tranditonal surgery, laparoscopic surgery had many advantages, such as the minimal invasion on the operative animals, excellent visualization, the less bleeding, postoperative mild pain, the less tissue adhesions and rapid recovery. It had been widely used in the domains of diagnosis and treatment of animal clinical diseases, livestock production, embryo transfer abroad. Abomasum fistula surgery is an effective way to collect abomasum content, analysis the extent of abomasum digestion, the escaped protein from rumen, the parasite in the abomasum by the installed fistula on abomasum. Now, experimental study about laparoscopic abomasum fistula surgery on sheep has not been reported in domestic and abroad, therefore, the purpose of this study is to design and perform the laparoscopic abomasum fistula surgery in sheep and to establish the surgical method, assess the technical feasibility and safety, and compared to the open abomasal fistula surgery in sheep. Through the influence of operation on physiological function, stress response and postoperative pain scores to evaluate the surgical results and further evaluation of the feasibility of laparoscopic abomasum fistula surgery in sheep, to provide the theory for the development of laparoscopic surgery.In this experiment, 12 local sheep were randomly divided into two groups, the laparoscopic abomasum fistula surgery group and the open abomasal fistula surgery group. Laparoscopic group chose the method of "three holes" by establishing of pneumoperitoneum, entering into cannulas and instruments, placing the T-latex fistul at the bottom place of the abomasum, making the purse-string suture, pulling retention suture on both sides of the abomasum to make the abdominal wall adhesions with abomasum. The open abomasal fistula surgery group finished the experiment by exposing the abdominal cavity, pulling retention suture on both sides of the abomasum, placing the T-latex fistul at the bottom place of the abomasum, making the purse-string suture ,fraping the retention suture, making the abdominal wall adhesions with the abomasum. During the surgery, monitoring the clinical signs and physiology index in sheep, collecting venous blood to test the stress response and liver-kidney function, and grading the postoperative VAS pain to evaluate the pain response of the two operations. After 20 days, technical feasibility of laparoscopic abomasum fistula surgery in sheep were evaluated by laparoscopic exploration, and observed adhesions.Laparoscopic abomasum fistula surgery was successfully completed with the mean operative time of 49min, and the average length of incision of 2.6cm; the open abomasal fistula surgery was completed with the mean operative time of 43min, and the average incision length of approximately 10cm. The heart rate, body temperature, blood pressure and respiratory rate were all decreased and end-tidal partial pressure of carbon dioxide increased in both group after the start of surgery, but they all recovered after surgery gradually. Compared with the open abomasal fistula surgery, laparoscopic abomasum fistula surgery had a slight effect on respiratory system and circulatory system. Although there was no significant difference on hepatic and renal function between the two groups, the open abomasal fistula surgery had a greater impact on the body. Postoperative pain scores in the laparoscopic group was the highest 6h after operation, then decreased gradually. The open abomasum fistula surgery group increased 6h after opertion, 12h after surgery to achieve the highest, and then began to decrease. In the laparoscopic group, animals recovered more faster.Compared with open abomasum fistula surgery group there was a lighter influence on the body in the laparoscopic abomasum fistula surgery group. Collected the content of the abomasum after 1 week, both of the two groups were succeed. 20 days after the opertion, do the laparoscopic exploration, abomasum and body wall formed a solid adhesion in all the sheep.Through the experiment results, the following conclusions were drawn:1. Laparoscopic abomasum fistula surgery in sheep was accomplished successfully with the technique of laparoscopes, and the fistula fixed definitely, the content of the abomasum was collected well after operation and quickly recovery. This show: laparoscopic abomasum fistula surgery is a safe, effective method and deserved generalization.2. And compared with the open abomasum fistula surgery in sheep, laparoscopic surgery has many advantage, such as excellent visualization, the less bleeding, little effect on the physiological function of the body, less stress response, postoperative mild pain and rapid recovery, etc.Thus, laparoscopic abomasum fistula surgery was a better surgical method.