Comparison And Evaluation Of Blood Metabolic Profiles In Dairy Cows With Letf Displacement Of Abomasum

Dairy cows suffer from a series of physiological changes, in order to support theneeds of pregnancy, calfing and lactation during the periparturient period of dairycows. Especially, in the late pregnancy and early lactation stage, the demand forenergy and the physiological changes are particularly intense. The perinatal cowsdevelop immunosuppression, resulting in metabolic and infectious diseases. Leftdisplaced abomasum (LDA) of dairy cows, one of the most common diseases ofperiparturient cows, is a multifactorial disease, mainly in high production dairy cows,and it is still quite common to occur at1~2weeks after calfing. LDA contributes tothe reduce of milk production，culling and death of cows, which has a tremendousimpact on dairy cow industry. Therefore, it is necessary to carry out the herdmonitoring of the disease in scale dairy farm. Using “campton metabolic profiles”, aviewpoint and theory of preventive medicine by clinical examination, by detectingtermly some components in blood, urine and milk could evaluate nutritional andmetabolic status of individual and herd, and warn an occurrence of metabolic diseases.And then some advice and guidance on feeding and management of the dairy cowscould be made.We measured the energy metabolism-related indices, liver and kidney function,immunology indices, the concentrations of mineral element analysts in healthy andLDA cows using ELISA and biochemical methods, and compare the diagnostic utilityof relevant indicators with receiver operating characteristic curve (ROC cure). Theresult showed that essentially all LDA cows displayed glucolipid metabolic disorderresulting from negative energy balance, affected normal function of liver and kidney,and decreasing insulin sensitivity. The concentrations of calcium in blood (cut-point:2.25mmol/L) are the major index for monitoring LDA cows. Some reference indexesfor diagnosing LDA and herd monitoring, include INS, RQUICKI, NEFA, ALT, GGT,CREA, AST, ALT, Cl and K, and their cut-points were calculated. Compared with healthy cows, the LDA cows showed a significant increasing concentrations ofcytokines and acute phase proteins, such as IL-1, IL-2, IL-6, IL-8, IL-10, TNF-α, CRP,CP, HP, SAA, C1q, and α1-AGP, indicating there is inflammatory reaction in LDA, andplay the important roles in the monitoring and diagnosis of LDA.In conclusion, periparturient dairy cows with LDA experience negative energybalance and negative calcium balance, and a certain extent of liver and kidneydisfunction, insulin resistance and inflammatory reaction. Combined indicators areconsidered to be useful for monitoring of LDA in scale dairy farm..