Study Of Laparoscopic Biliary Drainage In Sheep

Compared with traditional laparotomy, laparoscopic surgery has less bleeding, minimal trauma and quickly recovery features. Laparoscopic techniques had been widely applied in veterinary field abroad, for example, veterinary clinics, livestock production, embryo transfer and so on. However, application of laparoscopic techniques in animal fields was few in China.Sheep-bile showed markedly inhibiting action to acute and chronic inflammation. At present, the obtaining of sheep-bile was mainly by the removal of the cholecyst after being slaughtered. Also had some reports about open biliary drainage in sheep, but there was some impact to body of animal, because the location of cholecyst hardly exposed and it was difficult to tow. Therefore, we applied laparoscopic techniques to biliary drainage of sheep, and validated safty and feasibility of it.Comparison of two surgical methods, which can be provide the scientific basis for laparoscopic technology in veterinary.12 healthy sheep were selected and randomly divided into two groups, Laparoscopic biliary drainage group and Open biliary drainage group. Through putting trocar to finish pneumoperitoneal without veress needle, entering into cannulas and instruments, tractate cholecyst and installed the fistula to perform laparoscopic biliary drainage. It was monitored during the operation with intensive care monitor and respiratory monitor and were recorded at the time of before the operation, after anesthesia 10min, 30min and postoperative immediately, and then analyzed the physiological impact which surgery produced. Through open the peritoneal cavity, explored the cholecyst and installed the fistula to perform open biliary drainage, it was monitored during the operation, too. Pain state of sheep and effect of bile collected were observated after operation and was explored abdominal adhesions at postoperative 20 days. The venous blood of the neck at the multiple time points after surgery was collected in order to detect stress.Two operations were completed successfully, the laparoscopic biliary drainage with the mean operative time of 50min, the average incision of 2.5cm, the open biliary drainage with the mean operative time of 44min, the average incision of 12cm. Intraoperative blood pressure, heart rate, respiratory rate and pulse oxygen saturation of two groups showed the trend which first decreased and then increased, and the open group have more change in the trend than the laparoscopic group. Intraoperative laparoscopic end-tidal carbon dioxide partial pressure was significantly higher than the open surgery group. Drainage of bile reached the expected effect, either laparoscopic group or open group. VAS pain scores of the sheep which during the 3 days after surgery was the open group significantly higher than the laparoscopic group, and the open group was significantly higher than the laparoscopic group in the degree of adhesion. Comparison of the effects of the two kinds of surgery on the stress hepatic and renal function, open biliary drainage had the greater effects. The effects of collected bile in all sheep were good.In this study, successfully completed the laparoscopic biliary drainage in sheep, during operation, respiratory and circulatory system was monitored and had not adverse reactions and the effects of biliary drainage were good. This showed: laparoscopic biliary drainage was a safe and feasible technique.Compared with open biliary drainage, laparoscopic biliary drainage in sheep had advantages of minimal trauma, clear vision, less bleeding, mild pain and rapid recovery, in addition, postoperative abdominal adhesions in laparoscopic group was significantly less than the open group level, it is means there is a little effect on the body in laparoscopic biliary drainage.