Pap Promote The Induction Of Bone Marrow Mesenchymal Stem Cells To Neuron-like Cells Transformation Test

Purpose: Observation pilose antler many Maos(Velvet antler polypeptides, VAPs) divide into the influence of the biology behavior of nerve dollar kind cell on the inducement outside the body of marrow Chong quality stem cell(mesenchymal stem cells, MSCs) under the cell and the member level.Thus for pilose antler many Maos are better Be applied to clinical provided a theory basis, provide new path for paroxysm treatments like nervous system,etc.Method: At without under the germ condition make use of gradient density leave the heart method is from Wistar the big rat marrow separated a list pit the cell carried on original generation development, and passed change a liquid spread generation clean non- MSCs, put the third generation MSCs that purely turned into a development bottle, with bFGF+EGF carried on inducement, and respectively joined the different pilose antler for measuring many Maos(20 ugs, 40 ugs, 80 ugs) associately induce, and with joined standard development the liquid set carried on to check against a research, respectively after inducing of 1, 5, 7 ds, at set upside downed a microscope the bottom observed the appearance of each MSCs learn variety, 24 hs after just deeply shot give or get an electric shock a mirror the bottom observed induce after the variety circumstance of each MSCs.Finally examine an each MSCs cell Diao Wu circumstance with the flow type cell instrument(FCM), and with confirm the different measures of pilose antler the influence of many Maos upon the cell Diao Wu.Result: Prepare induce after developing a 24 hs, the cell number of appearances have no obvious variety, still take shuttle form or expanse flat even form as a lord, join to induce an EGF+BFGF+after many Maos induce a 5 ds, the pilose antler's taking place of cell obvious appearance learns variety, majority of cell changes rise for being similar to double very or much and very form nerve dollar kind cell appearance, stretching out being similar to axon or dendrite kind Tu.The flow type cell instrument examination cell Diao Wu circumstance, pilose antler many Mao set compare bFGF+EGF and standard develop liquid set the Diao Wu rate decrease, the big measures compare obvious difference with blank matched control more obviously. Conclusion: 1, pilose antler many Maos can induce outside the body MSCs to divide to the nerve dollar kind cell.2, Different the pilose antler for measuring many Maos induce MSCs converts to the nerve dollar kind cell, and leads to the cell Diao Wu of the influence is each not same .