Pap Induced Bone Marrow Mesenchymal Stem Cells Into Neuron-like Cell Transformation To Promote

Objective: This article is a study of pilose antler peptide on the bone marrow mesenchymal stem cells induced with or without promoting by cell biology and molecular biology. It provides more theoretical basis for the clinical application of PAP,and provides a new therapeutic approach for repair of spinal cord injury.Methods: Percoll isolated from Wistar rat bone marrow monocytes were cultured to passage through the medium change unless the MSCs, has been purified MSCs. Containing bFGF + EGF medium basis, and different doses of PAP were added for induction, and did not join the PAP, but only with bFGF + EGF medium were the basis of the induced group and only increase the standard culture medium as control study. First to the MSCs morphology observation and the correlation detection, and carries on the analysis to its characteristic. Then after inducing the cell carries on the morphology observation and the index of correlation examination analysis.Results: The MSCs original generation of cell pastes the wall to grow, which shape likely spindle; The transfer of generation raise, the shape becomes the ciliary cell type, assumes spindle-shaped. Flows the type cell meter result to demonstrate: In 3rd generation of MSCs the G0/G1 time's cell is 75.97%, conforms to the stem cell characteristic; Induces MSCs to the osteoblast differentiation. Thus proved that these cells have the multi-direction split up potential, conforms to the marrow mesenchyma stem cell characteristic. after bFGF+EGF or the bFGF+EGF+ velvet multi-peptides induce 1, 5, 7d, the majority cells transform into similar double-pole or the multipolar neuron type shape, stretches out the similar axon or the tree type breaks out suddenly; The blank control group cell change are few. The RT-PCR result showed: After the induction, cell GDNF mRNA has the varying degree expression, but the blank control group cell GDNF mRNA expression is weak. After inducing 7d, carries on the immunocytochemistry dyeing to demonstrate: The induction group NSE dyeing assumes the varying degree masculine gender, the blank control group assumes the weak masculine gender; But joins the velvet multi-peptide different monitoring team to compare other group of obvious somewhat, joins the velvet multi-peptide 40ug/ml induction group to compare other four groups to be more obvious.Conclusion:1 PAP MSCs in vitro to promote neuron-like cells induced to differentiate.2 MSCs induced GDNF expression, suggesting that GDNF involved in MSCs into neuron-like cell differentiation.3 GDNF gene was activated explain PAP may have induced.