Independent Living Experimental Study On The Role Of Immune Injury Intervention In Alzheimer's Disease Rat Model

Purpose: To investigate the mechanisms of Duhuo to delay cerebral aging through studying inhibitory effect of Duhuo water granula on immune injury in AD model of Wister rats so as to provide experiment data for study of cerebral aging.Material and method: The use of Aβ-amyloid protein injection method of Wister rats and Hippocampus to shape AD model, traditional Chinese medicine Danshen, Western medicine indomethacin, and saline were administered. After four weeks, effect of drugs on learning and memory in rats is observed through the water maze experiment; ELISA method is used to detect expression of cytokines in serum IL-4 and TNF-αin different groups.Results: The content of IL-4 in AD model Wister rats was lower (p<0.01) than that of general Wister rats, whereas the content of TNF-αwas higher (p<0.01) . Compared with model group, IL-4 secretion in the Chinese medicine group was higher (P <0.01). whereas the content of TNF-αwas lower (p<0.01) .Conclusion:1. Duhuo could inhibit the inflammation of central nerves of AD model Wister rats.2. Duhuo water granula could delay cerebral aging the mechanism is to regulate immune function so as to prevent and trent immune injury.