| Purpose: Anal disease postoperative pain is a typical performance, many patients in theclinical, he was afraid because of the severe pain of anal disease after refused surgicaltreatment or delayed treatment, and thus missed the best time of the treatment of the disease.Currently, for anal disease postoperative patients, clinical commonly used pain medication asthe primary means of resolving pain. In recent years, the clinical application of the painkillerindeed achieved good results, but some of the side effects of drugs and drug dependence aremore obvious, such as nausea, vomiting and other gastrointestinal discomfort various oralanalgesics after.Acupuncture is an important part of Chinese traditional medicine, for thousands of years,acupuncture has been used to treat various diseases. Ancient literatures, acupuncture pain firstvisible in the changsha mawangdui silk "meridians" found in the writing, including records ofacupuncture and moxibustion can eliminate "have a headache, backache, abdominal pain,back pain, joint pain," and other kinds of pain diseases. Huangdi neijing is detailed discussedthe etiology and pathogenesis of all kinds of pain, and established the principle of acupunctureanalgesia, acupuncture analgesia has a guiding significance on later generations. In recentyears, with the continuous improvement of the related theory, acupuncture anesthesia andacupuncture analgesia play a more and more important role in the medical field. Ourdepartment obtained through clinical trials and Practice for many years, Electro-acupunctureBaihuan Yu can effectively relieve postoperative pain of anal disease, And found in the clinicaltest of Electro-acupuncture Baihuan Yu and analgesics, the utility model has the advantages offast onset of action, analgesic effect for a long time, no side effect of Electro-acupunctureBaihuan Yu in the postoperative analgesia.Now the understanding from the Angle of science of acupuncture analgesia, The sharpcut in rats caused by the anus anal postoperative model, to observe the effect ofElectro-acupuncture Baihuan Yu effect on the concentrations of NO, SOD in serum of rats,Discuss the mechanism of acupuncture analgesia from molecular biology and oxygen metabolism,and experimental results calculated using statistical approaches and analysis of the dataconcluded that in order to clear the mechanism of analgesia of Electro-acupuncture Baihuan Yu. Method: Wista rats were divided into two groups,30rats in each group, half male andhalf female, were divided into electro-acupuncture group (group A), control group (group B)of two groups. A longitudinal incision of1.5cm caused the anus anal postoperative model.Group after10minutes in the ocular fundus blood2ml, and marked as A1sample, the sampleis placed in the-20℃device.30minutes after modeling, rats given A bilateralelectro-acupuncture Baihuan Yu, to release the gas needle method,14-26/min dilatationalwave given point stimulation30minutes. The whole process requires fixed rat rat, if notproperly adjusted to an electro-acupuncture in rats. Upon the end of the Electro-acupuncture withsyringe in the rat fundus blood1ml and marked as samples A2. Blank group after10minutes in the ocularfundus blood1ml, and marked as B1sample, the sample is placed in the-20℃device. After60minuteswith a syringe in the rat fundus blood1ml and mark for the sample of B2. When detecting, removal of theblood samples,3000to10min/min centrifugation, separation of serum, the OD value and concentrationwere detected by immunohistochemistry NO, SOD in serum samples. The blood remaining rats using thesame method to detect the concentration of NO, SOD.Result:1, Electro-acupuncture ago, two groups of rats, serum NO, SOD concentrationcompared statistically no significant difference (P>0.05).2, After electro-acupuncture, compare NO, SOD concentration in serum of rats in twogroups:(1) Comparison of the concentration of serum NOAfter electro-acupuncture acupuncture30min, electro-acupuncture group (group A) inthe serum concentration of NO than the control group (group B) NO concentration in theserum decreased significantly, the use of statistical processing two sets of values, there aresignificant differences (P<0.01).(2) Comparison of the concentration of serum SODAfter electro-acupuncture acupuncture30min, electro-acupuncture group (group A) inthe serum concentration of SOD than the control group (group B) SOD concentration inserum increased significantly, the use of statistical processing two sets of values, there aresignificant differences (P<0.01).(3) before and after acupuncture group acupunctureAfter electroacupuncture acupuncture30min, EA group (group A) in the serum concentration of NO than the control group (group B) NO concentration in the serumdecreased significantly, the use of statistical processing two sets of numerical, has a verysignificant difference (P<0.01); Electro-acupuncture group serum SOD concentrationcompared with blank control group, serum SOD concentration significantly elevated, usingstatistical processing two sets of numerical, has a very significant difference (P<0.01).(4) the blank group before the experiment and after the experimentSerum NO concentration compared with experimental serum NO concentration in the blankcontrol group before the experiment decreased use of statistics dealing with two sets of values,there is a significant difference (P <0.05). The blank control group before the experiment of serumconcentration of SOD than the SOD concentration in serum increased after the experiment, the useof statistical processing two sets of values, there were significant differences (P<0.05)Conclusion: The electro-acupuncture group (group A) and control group (group B) foundthat electro-acupuncture and electro-acupuncture Baihuan Yu can be adjusted, concentration ofNO and SOD in serum of rats. Blank control group by group comparison, found that the contentof NO in serum of rats decreased after the experiment, serum SOD content increased.Afterelectro-acupuncture stimulation, NO concentration in serum of rats decreased significantly,and the concentration of SOD increased significantly, while that in the inhibition of NOsynthesis of electro-acupuncture, can also promote the formation of SOD. |
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