Matrine On In Vitro Cell Proliferation Of Pterygium Fibroblasts

OBJECTIVETo reduce the necessity of surgical intervention, invest the influence of Matrine (Mat) on the proliferation of cultured human pterygium fibroblasts (HPF), and discuss a new method to pterygium drug treatment clinically.METHODS1. Human pterygium fibroblasts (HPF) were cultured in vitro with explants culture method, and the cultured cells were identified by morphological examination and the hematoxylin-eosin, HE straining and immunohisto-chemistry SP staining techniques.2. Colorimetry MTT was used to detect viability of Matrine in different concentra -tion (0.1, 0.2, 0.4, 0.8,1.6, 3.2, 6.4 and 12.8mg/ml) at different time (24, 48 hours) for inhibition of proliferation of HPF, and calculated the 50% inhibitory concentra -tion (IC₅₀) of Matrine.3. Mitomycin C(MMC)as the masculine comparision group, compared the inhibit -tion effects of the Matrine with Mitomycin C.RESULTSAdministration of 0.4～12.8mg/ml Matrine for 24～48h could significantly inhibit HPF proliferation in a dose dependent manner (P<0.05). There were obvious inhibitory effects of Matrine on HPFs in the concentration of 0.4mg/ml for 24 hours (P<0.05), in the 50% inhibitory concentration (IC₅₀) of Matrine was 7.9190 mg/ml and more evident in the concentration of 0.4mg/ml for 48 hours (P<0.01) compared with control groups, in the 50% inhibitory concentration (IC₅₀) of Matrine was 1.8115mg/ml. Compared with Mitomycin C, the inhibitory proliferation effect is inferior.CONCLUSIONCultured HPFs appear to be obvious proliferative activity. There is a remarkable inhibitory effect of Matrine on HPFs, and the effect shows relativity with time and dose. This experiment provides a new method to cure and prevent the first pterygium and recurrent pterygium.