| Objective: Observation juvenile rat model of central and peripheral cholecystokinin octapeptide (CCK-8) and B-endorphin (β-Ep) content, and explore Kaiweixiaoshi Syrup on central and peripheral CCK-8β-Ep and the regulatory role and observe their appetite changes.Methods : Wistar rats 30, at the age of 35-40 days, weight 60g＋10g, half male and half female; were randomly divided into three groups (blank group, model group, Kaiweixiaoshi Syrup treatment group), 10 in each group. Apart from the normal group was fed a normal diet, the other two children simulation of the main causes of anorexia, Wang Chuan-use simulation etiology (Nanjing University of Traditional Chinese Medicine Act customized feed model) Preparation of special feeding rats Making juvenile rats. All rats were fed individually, the free flow. Raising a total of five weeks. Rats record daily food intake and body weight in model rats feeding decreased 40-60%, weight lower than the control group 10-15, but no diarrhea, Lordiphosa, crouching behind such notable spleen deficiency symptoms successful model signs. The treatment group was kaiwei Xiaoshi syrup for four weeks, one a day. The other two groups do use saline control. Experimental around the course, the rats feeding volume changes; Around the end of the experiment, the rats were to be taken from the blood and gastric mucosa from some organizations and the hypothalamus, RIA using detection technology to measure the animals central and peripheral CCK-8 and Chinaβ-Ep content the same time using some of antral mucosa Preparation HE staining slides, the rats gastric tissue pathology.Results: Group hypothalamus and plasma CCK-8 was significantly higher than the normal group (P＜0.05, P＜0.05) treatment of hypothalamic and plasma concentrations of CCK-8 model was significantly decreased (P＜0.05) and the normal group was not significant (P＞0.05); model group hypothalamusβ-Ep with the normal concentration was not significantly different (P＞0.05), antral mucosa and plasma concentrationβ-Ep significantly lower (P＜0.001, P＜0.05, treatment group and the hypothalamusβ-Ep antral mucosa were significantly higher than the model group and the normal group (P＜0.001), Plasma concentrationβ-Ep model group (P＜0.05) Compared with the normal group had no significant difference. Model animal food consumption decreased for three weeks or more. Feeding the treatment group during the first three weekends and 4 weekend compared with the normal group had no significant difference, normal rat gastric mucosa organizations, submucosa, muscularis no infiltration of inflammatory cells, rat model mucosal surface of epithelial cells, lamina are high neutrophil infiltration, Some models can be seen in rats mucosal surface epithelial cells necrosis, kaiweixi Xiaoshi syrup after gastric tissue in rats lamina neutrophil infiltration has declined markedly.Conclusion: Kaiwei xiaoshi Syrup is the clinical use of the prescription for many years, are to follow "for peace Take for health" aperitif YUNPI the basic rules to Win Chinese consumer's agent solution temper the importance of saving money allocated Ling dirty gas, stomach reconcile, Rehabilitation spleen operation, the largest Be selective.The experimental results suggest that juvenile rats central and peripheral CCK-8 concentrations increased; juvenile rat model of peripheralβ-Ep reduce the concentration, the concentration of centralβ-Ep no significant impact; mucosal surface epithelium, lamina are high neutrophil infiltration, some anorexia rat model even the mucosal surface of epithelial cells necrosis. The treatment group Kaiweixiaoshi Syrup after treatment can significantly promote juvenile rats central and peripheralβ-Ep secretion, Inhibition central and peripheral CCK-8 secretion and improve its stomach inflammation changes, thereby contributing to feeding behavior, increased appetite. The experimental results are reported in the literature and inconsistent. So that CCK-8β-Ep with the central and peripheral to the imbalance is to reduce animal feeding one of the reasons with the current coverage of the literature basically the same. Kaiweixiaoshi Syrup can adjust CCK-8β-Ep with the central and peripheral ratio imbalance improve gastric tissue inflammation change, the treatment of anorexia may be one of the mechanisms. |
PDF Full Text Request