The Experimental Study Of Shugan Rationale Spleen Soup For Losses Of Appetite The Model Big Mouse Of Cholecystokinin Octapeptide, β-endorphin Influence

Objective: To investigate " Sparse liver principle spleen soup " of anorexia in rats food intake, body weight, hypothalamus and plasma CCK-8,β-EP effects of the treatment of children to explore the mechanism of anorexia and clinical application to provide experimental basis.Methods : 50 SD rats were randomly divided into normal group, model group, Xiaoshi film group, high-dose group, low-dose group, 10 in each group, the causes of anorexia in rats simulation model to copy. Model is successful, the experiment started the 8th day oral treatment, fasting 8:00 the evening of the 28th, the first executed on the 29th, blood, hypothalamus, the index measures.Results : (1) The impact of food intake: a successful model after the first 14 days of the experiment, high-and low-dose group was significantly higher than the model group, compared with the significant difference (P <0.01, P <0.001); the first 21 days of the experiment, Xiaoshi film group was significantly higher than the model group, compared with a significant difference (P <0.001); At the same time, high-and low-dose group was higher than Xiaoshi chip group, compared with significant difference (P <0.001); high-dose group was higher than that of low-dose group, compared with a significant difference (P <0.05). The first 28 days of the experiment, high-dose group was higher than that of low-dose group, two more significant difference (P <0.01); low-dose group compared with the Xiaoshi chip and high-dose group and normal group were not significantly different (P > 0.05). (2) The impact of body weight: the first 14 days of the experiment, the model group, normal group Xiaoshi film group, compared with a significant difference (P <0.01); Xiaoshi tablet group compared with the model group was no significant difference (P> 0.05); high , low-dose group was higher than Xiaoshi film group, compared with significant difference (P <0.05); high-and low-dose group there was no significant difference (P> 0.05). Experimental section 21,28 days, high-and low-dose group and Xiaoshi film group was higher than the model group, compared with the significant difference (P <0.001, P <0.05); high-dose group was higher than Xiaoshi film group, compared with There was significant difference (P <0.01); high-dose group was higher than that of low-dose group, compared with a significant difference (P <0.01); low-high dose group compared with the film Xiaoshi no significant difference (P> 0.05).(3) Of the hypothalamus and plasma CCK-8 content: After treatment, high-and low-dose group was significantly lower than the film Xiaoshi model group, compared with a significant difference (P <0.001); high-and low-dose group was significantly lower Xiaoshi films in groups, with more significant differences (P <0.05); high-dose group was lower than that of low-dose group, two groups were significantly different (P <0.05); high-dose group and normal group there was no significant difference (P > 0.05).(4) Of the hypothalamus and plasmaβ-EP content: After treatment, high-and low-dose group were significantly higher than that Xiaoshi chip model group, compared with a significant difference (P <0.01, P <0.001); high-and low - dose group was significantly higher than Xiaoshi chip group, with more significant differences (P <0.001, P <0.01); high-dose group was higher than that of low-dose group, compared with a significant difference (P <0.05); high-dose group and normal group there was no significant difference (P> 0.05). Conclusion : " Sparse liver principle spleen soup " could significantly promote anorexia in rats food intake, weight gain, its mechanism may be through the reduction of animals in the hypothalamus and plasma CCK-8 levels increased in hypothalamus and plasmaβ-EP concentration to achieve the .