Spleen And Kidney And Fill Method On Aging Mice Induced By Immune Function

ObjectiveThis essay established senile model induced by injection of back D-gal to rats .Through the pharmacology and immunology research technique. This experiment is to studied the effect of "pi shen shuang bu " therapy on change of SOD ,MDA, IL-2, CD28 for analyzing the mechanism and action of this therapy for senility. The effects of PSSB therapy on oxygen-radical-generated DNA damage in senile rats spleen and chest gland cells .Discuss the effects of this therapy for delay senility. This will provide new prevention and cure for old age diseaseMethodsSixty normal rats,2-months in age,20±2g, randomly divided into six groups: normal control group, model group, high dosage PSSBP-treated group(high dosage group),middle dosage PSSBP-treated group(middle dosage group), low dosage PSSBP-treated group(low dosage group)and VitE medicine control group.Through with single cell gel eletrophoresis (comet assay) studied the effects of PSSB therapy on oxygen-radical-generated DNA damage in senile rats spleen and chest gland cells .Detection the content of SOD MDA in spleen , chest gland and level of IL-2,CD28 in spleen.ResultsThe rats′comet cell tail length between model group and spleen normal group had the remarkable difference (P<0.01) .Spleen cell in VitE medicine control group and every dosage PSSBP-treated group between the model group had the remarkable difference (P<0.01). Compared with VitE medicine control group, middle dosage group and high dosage group had the remarkable difference (P<0.01). Low dosage group did not have difference .Compared with high dosage group, middle dosage group and low dosage group had the obvious difference (P<0.01). The rats' comet cell tail length between model group and chest gland normal group had the remarkable difference ( P<0.01 ) .The comet cell tail length in middle dosage group and high dosage group had the remarkable difference( P<0.01 ), VitE medicine control group and low dosage group had the difference ( P<0.05 ) , Compared with VitE medicine control group, chest gland cell in high dosage group had the remarkable difference( P<0.01 ), middle dosage group had the difference ( P<0.05 ) , low dosage group had no difference.Compared with high dosage group, low and middle dosage group had the remarkable difference( P<0.01 ).The content of SOD in spleen cell between model group and others group had the remarkable difference( P<0.01 ). Compared with VitE medicine control group, middle dosage group and high dosage group in spleen cell had the remarkable difference( P<0.01 ),low dosage group had no difference . Compared with high dosage group, spleen cell in middle dosage group had the difference ( P<0.05 ) , low dosage group had the remarkable difference( P<0.01 ). Compared with model group, chest gland cell in every group had the remarkable difference ( P<0.01 ).Compared with VitE medicine control group, chest gland cell in high dosage group had the remarkable difference( P<0.01 ), middle dosage group had the difference ( P<0.05 ) , low dosage group did not have difference. Compared with high dosage group, low dosage group had the remarkable difference( P<0.01 ), middle dosage group did not have obvious difference.The changes of MDA in model group was higher than the other groups in spleen cell obviously( P<0.01 ), compared with VitE medicine control group, middle dosage group and high dosage group had the remarkable difference( P<0.01 ), low dosage group had the difference ( P<0.05 ) , Compared with high dosage group, low dosage group had the remarkable difference( P<0.01 ) middle dosage group had the difference ( P<0.05 ). The changes of MDA in model was higher than the other groups in chest gland cell had the obvious difference ( P<0.01 ), compared with VitE medicine control group, middle dosage group had the difference (P<0.05) , high dosage group had the obvious difference ( P<0.01 ), low dosage group did not have obvious difference. Compared with high dosage group, low dosage group had the remarkable difference (P<0.01), middle dosage group did not have obvious difference.The level of IL-2 in model group, high and middle dosage group were obvious higher than model group( P<0.01 ), VitE medicine control group and low dosage group had the difference ( P<0.05 ). Compared with VitE medicine control group, high group and middle dosage group had the difference ( P<0.05 ) low dosage group did not have obvious difference. Compared with high dosage group, low dosage group had the remarkable difference (P<0.01), middle dosage group did not have obvious difference.The level of CD28 in model group, high and middle dosage group were obvious higher than model group( P<0.01 ),VitE medicine control group did not have obvious difference. Compared with VitE medicine control group, high group and middle dosage group had the obvious difference ( P<0.01 ), low dosage group had the difference ( P<0.05 ) Compared with high dosage group, middle and low group did not have obvious difference.ConclusionThis experiment through with single cell gel eletrophoresis (comet assay) studied the effects of PSSB therapy on oxygen-radical-generated DNA damage in senile rats spleen and chest gland cells. The result showed that the Chinese native medicine may reduce tail length of the comet cell and offered significant DNA protective effects. Obviously increase the DNA protective effects on senile process and delay senility.After the treatment ,the Chinese native medicine may increase the level of SOD in model group , The result showed that SOD may increase capability of eliminate Free Radical and delay senility. The Chinese native medicine may reduce the level of MDA and delay senility.After the treatment, The Chinese native medicine may increase the activity of IL-2 in senile organism. Through increase the IL-2′important immunity function and then delay senility.The experiment showed that the level of CD28 in model group was lower than normal group. The result may showed that senility reduce CD28′important immunity function in activity of T cells. The Chinese native medicine may increase the level of CD28.The mechanism of this may that CD28 have very important in regulating aged T cell function, and then delay senility.