Gavage Gynostemma Extract The Natural Aging Mice Skin Tissue, H <sub> 2 </ Sub> O <sub> 2 </ Sub> Content, Sod Activity In Experimental Research

Objective:Andstudy irrigation stomach traditional Chinese medicine wrings share blue pick up liquid organizing the activitybeing hit by perhydrol (H₂O₂) contents , superoxide dismutase(SOD) to small rat skin at ease oldand feeble, small rat skin ultrastructure effect, judge that skein of share is blue in the effect, theory and modern medicine old and feeble knowing and expounding and proving doctor of traditional Chinese medicine to add supplemental false strengthening the body resistance resisting lightening natural small rat skin ageing aspect about that skin aging free radical injures theory picking upliquid.Methods: March kind of healthy Kunming of age small rat 10 is youth contrast a group (A); 40 age small rats in December at ease old and feeble the random divides 4 set: Old age takes blank space orally contrasting the group (B), old age takes masculine gender orally contrasting the group (C), old age gives medicine the group (D) for oral administration, old age (for oral administration + for external application) gives medicine a group (E). A group, B group among them irrigate the stomach normal saline respectively , C irrigates Wei Sheng Su E the stomach set , the D group, the E groupspread skein of blue share frost besides irrigating the stomach skein of pick up liquid, simultaneous blue share E group respectively. The ultrastructure observing small rat skin organization changes 30 Heaven's Queen, analysis method determines and H₂O₂ contents and SOD activity with biochemistry.Results: Comparing with youth collation group, that SOD activity reduces other four old age small rats of group skin without exception obviously in organization (is respectively P＜0.01, P＜0.01, P＜0.01, P＜0.05), that H₂O₂ contents increase by obviously (is respectively P＜0.01, P＜0.05, P＜0.01, P＜0.05), pathological mechanism section assumes state of skin old and feeble; Comparing with the group who takes blank space orally in the dry tree, that old age takes masculine gender orally contrasting a group, SOD activity increases height without exception obviously in the skin organization who takes skein of blue share group and the old age (for oral administration+for external application) small rat of skein of blue share group orally in the dry tree (is respectively P＜0.01, P＜0.01, P＜0.01), that H₂O₂ contents reduce (difference obviously is P＜0.01, P＜0.01, P＜0.01), the skin state old and feeble pathological mechanism section is demonstrated is better;Compared with the group who takes skein of blue share D orally exclusively, (the difference comparatively strong to the effect improving SOD activity and bringing down H₂O₂ contents with the E group who gives medicine way is P besides fitting at the same time P＜0.05, P＜0.05), degree of pathological mechanism photograph display it's improvement skin old and feeble is also stronger.Conclusions: Skein of share is blue pick up liquid having the effect delaying to small rat skin senility at ease old and feeble; The composition professing to be convinced is for external application skein of blue share be given to poisons way being able to play the damage old and feeble to delaying the skin ageing naturally to the small rat's role better. Whosemechanism may be that skein of share is on one hand blue have the effect eliminating a free radical per se; Skein of share is on the other hand blue can achieve antioxidation's purpose by improving SOD coming activity, play arrive at delay skin is old and feeble role thereby. Since inside and outside is the thickness with giving medicine way to be able to raise body inner medicine's concurrently, more beneficial to airframe makes use of to the medicine sorption.