Gynostemma Experimental Study, The Sod Activity In The Blood Of Mice And Inhibit The Ability Of ¡¤ Oh

Objective: To study the effect of the extraction of Gynostemma on the activities of SOD and the capacity of inhibiting·OH in blood of the naturally senile mice and observe the change of the tissue structure of naturally senile mice,and from the two aspects,of the clinical significance of reinforcing deficiency and surppot right and the theory leading to skin aging free radical loss theory in modern medicine presume the mechanism on the effect of Gynostemma on posponing the senescence. Methods :10 health kind Kunming female mice aged in four-month forming a group as youth (A); 30 the kind of Kunming female mice aged in thirteen- month randomly divided into 3 groups,10 ones per group:old age blank group (B),old age masculine matched group (C),old age Gynostemma Pentaphyllum group (D). Gave the group A,B NS 0.3ml/t intragastriclly , external use blank cream 0.5ml/t; Gave the group C VE 25mg/kg intragastriclly, volume of giving medicine 0.3ml/t, external use VE cream 0.5g/t; Gave the group D the extraction of Gynostemma 0.3ml/t intragastriclly, external use Gynostemma cream 0.5g/t.All above was handled 2 times per day.After 30 days by biochemistry analysis method determined the activities of SOD and the capacity of inhibiting·OHin blood ,and observed the change of mice skin organizational structure.Results:Comparing with the youth group, the activities of SOD in blood reduces obviously among the three old age groups (P﹤0.01, P﹤0.05, P﹤0.05 respectively), and it is weakened by obviously to the capacity of inhibiting·OH (P﹤0.01,P﹤0.01,P﹤0.05respectively); Comparing with old age blank group, old age masculine matched group and old age Gynostemma Pentaphyllum group can improve the activities of SOD (P﹤0.05,P﹤0.05respectively) and the capacity of inhibiting OH·(P﹤0.01,P﹤0.01 respectively). Conclusions:That the the activities of SOD and the capacity of inhibiting·OH in blood of the naturally senile mice is reduced.The extraction of Gynostemma can clean up the free radical by intragastric administration with external use and prove the antioxidant punch could reduce the damage to the skin of mice casusing by the oxidant.Then pospone the senescence of the skin.It also proves that reinforcing deficiency to strengthen vital Qi is one of the efficient means of resisting dermal-senescence.