Gavage Gynostemma Extract Experimental Study On The Effects Of Natural Aging Mice Skin Tissue Sod Activity, Mda Content

Objective: The study aims at studying skein of share is Gynostemma pentaphyllum extracting an impact of liquid over small mice skin organization middle Superoxide Dismutase(SOD) activity and Malondialdehyde(MDA) of the small mice skin organization contents at ease old and feeble, to observe a small mice's skin organization architectural change and different be given to poisons the approach improvement effect to small mice skin at ease old and feeble, come to check skein of share Gynostemma pentaphyllum extract the liquid effect in the respect of lightening natural small mice skin ageing, delay a senility thereby.Methods: 10 kind of Kunming age in three-month female small mice contrast a group as youth(A); 40 kind of Kunming age in twelve-month female small mice the random divides 4 group, 10 per group: Old age irrigates stomach blank space contrasting group(B), old age irrigates stomach masculine gender contrasting the group(C), old age irrigates the stomach wringing the Gynostemma pentaphyllum group (D), old age(irrigate stomach+skein of Gynostemma pentaphyllum group(E) for external application). And give A, B group of irrigation stomach Normal salines, 0.8ml/t respectively; C irrigates the stomaches Vitamin E, 0.2ml/t; D irrigates the stomach group wringing share Gynostemma pentaphyllum the 0.8ml/t picking up liquid; E irrigates the stomach skein of pick up liquid(dose the same as D group), simultaneous Gynostemma pentaphyllum outside Tu 0.5g/t skein of Gynostemma pentaphyllum frost agent group. After 30 days by biochemistry analysis method determination skin organization in SOD activeness and MDA content, and observe mice's skin organizational structure change.Results: Comparing with youth collation group, SOD activity reduces without exception obviously among the others four old age skin organization of group (P＜0.01, P＜0.05, P＜0.01, P＜0.05), that MDA contents increase by obviously (P＜0.01, P＜0.01, P＜0.01, P＜0.01); Comparing with old age blank space group, old age irrigates stomach masculine gender contrasting a group, old age irrigates the stomach wringing the Gynostemma pentaphyllum group of share and (irrigates+the stomach in the dry tree for external application) wringing the Gynostemma pentaphyllum group of share but improving SOD activity (P＜0.01, P＜0.01, P＜0.01), lessening MDA contents(P＜0.01, P＜0.01, P＜0.01); Compared with simple irrigation stomach skein of Gynostemma pentaphyllum D group, (P＜0.05, P＜0.05) comparatively strong to the effect improving SOD activity and bringing down MDA contents giving medicine way other than.Conclusion: Skein of share is Gynostemma pentaphyllum pick up liquid having the effect delaying to natural small mice skin senility; Profess to be convinced matching the effect using skein of Gynostemma pentaphyllum that kind to give a medicine way energy fairly good getting up other than ageing at ease to delaying small mice's skin. The former mechanism may be that skein of share is on one hand Gynostemma pentaphyllum have the effect eliminating a free radical perse; Skein of share is on the other hand Gynostemma pentaphyllum can pass the natural senility improving SOD coming to achieve antioxidation's purpose activity, delaying skin thereby. The sorption may be the airframe thereby more beneficial.