| Background:Acute lung injury(ALI) is one of the common clinical refractory complications, which specially occurs in heavy infections, various shocks, traumas and surgeries. And it has become one of the major causes of death for its poor prognosis. In spite of the great improvement in modern treatment technology and facilities, there is little effect upon the ALI therapy except the early mechanical ventilation. So it is worthy of seeking an effective treatment. ALI has multiple causes and complicated mechanisms. Recent studies show that the oxidative stress induced by a great deal of ROS(reactive oxygen species) which have been generated in the respiratory burst by the activated cells plays a large part in the mechanism of ALL The most important one is the free radical provoked by oxygen called OFR(oxygen free radical), which is the generic name composed of molecules, atomic groups and atoms out-orbited by single unmated valence electrons. The OFR is characterized as short longevity, active chemical property and its susceptibility of reacting with other mass to create new radicals and to trigger chain reactions. The OFR is unceasingly produced in normal bodies and plays an important role as killing microbes, removing the old dead cells and tumor cells , regulating inflammations and so on. Few OFRs could be seen in normal bodies and could be rapidly cleared away by SOD and GSH-px etc, so the OFRs are not easily accumulated in normal tissues and cells. In ALI, however, as agreat deal of PMNs are gathered in pulmonary, the respiratory burst and the xanthine oxidase and so forth will lead to multiple OFRs that is more than the body could eliminate. They will also do harm to the tissues and cells, while the pulmonary is one of the most vulnerable target organs. The OFR has powerful oxidative activity and could impair almost all the biomolecules especially in the way of lipid peroxidation. The biomembrane (including cell membrane, mitochondria membrane and lysosomal membrane etc.) contains abundant polyunsaturated fatty acid that is extremely easy to be attacked into various lipid peroxidation mass for the powerful oxidative activity of OFRs. It will lead to the decline of membrane flowability and add to the permeability , finally the malfunction of the biomembrane. At the same time the OFRs could do great harm to the protein, enzyme and nucleinic acid, and they may stimulate the transcription factor, induce the transcription and generate considerable cytokines and mediators of inflammation to participate in the lung injury. Hence, it will protect the ALI to find an effective OFR scavenger. Edaravone is a kind of powerful new oxygen free radical scavenger, which can protect the brain cell damage in ischemic reperfusion and has been widely used in the treatment of acute cerebral infarction. Some research discovered that the edaravone can act as a protection in all the disorders participated in by OFRs such as the kidney damage caused by DDP, the liver injury induced by ischemic reperfusion,myocardial damage in the diabetes and so on. While few reports could be seen about its protection in ALL So we made an tentative experiment in order to explore its function in ALLAnimal experimentObjective To investigate the anti- oxygen free radical friction of edaravone in ALI on big rats.Methods Rats were divided into four group randomly: control group (A), injured by oleic acid group (B), injured by oleic acid and prevention of edaravone group (C), injured by oleic acid and treatment of edaravone group (D). Assessed the level of oxygen free radicals by examining malondialdehyde( MDA) ,superoxide dismutase (SOD), r-glutamylcysteinylglycine (GSH) and glutathione peroxidase (GSH-px) in blood, assessed the pulmonary edema degree by calculating lung wet gry weight ratio and total protein in broncho-alveolar lavage fluid (BALF), the pathological slices were made from the tissues of apex of right lung and checked under light microscope.Results Compare with group A, MDA of others groups significantly increased (p<0.01), but MDA in group C and group D was lower than those in group B (p<0.05); Compare with group A, SOD, GSH and GSH-px of others groups were significantly increased, but those in group C and group D were significantly higher than those in B group (p<0.05); Total protein in BALF andlung coefficient in group C and group D significantly decreased than those in group B (p<0.01);HE staining showed that the lung injury in group C and group D was improved.Conclusion1. Edaravone can decrease oxyradicals effectively on acute lung injury of big rats by oleic acid.2. Edaravone can improved pathological slices of on acute lung injury.3. Edaravone has protective effects on on acute lung injury of big rats...
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