The Modified Zhizhu Tang Experimental Spleen Deficiency Syndrome And Rat Il-1 And D-xylose

Objective:Study an old Chinese medicine experience prescription" Add Medicine Zhizhu soup " , discuss the effect on IL-1 and D-xylose,clarify the material foundation that Add Medicine Zhizhu soup cures insufficiency of the spleen-qi.The first step study the relationship between insufficiency of the spleen-qi and the nerve- endocrine- immunity network ,and research the effect of Add Medicine Zhizhu soup for this network, thus announce to public the whole function . Establishes the good foundation for urther development of this product.Method:1, Theories research: Take the research towards Add Medicine Zhizhu soup in each drug two aspects,①Elaboration of ancient times Chinese doctor,②Modern pharmacology research (the contents limit at cure Insufficiency of the spleen-qi and digesting system, the influence of the nerve, immunity, the endocrine system); 2, Experiment research: Take 60 of Wistar rat, and divided into Normal group, Insufficiency of the spleen-qi Model group, Add Medicine Zhizhu soup Large dose group, Center dose group and Small dose group. 12 per set. In addition to the normal group, the other groups adopts Chaotic diet, Excessively tired and a little amount of bitter and cold drug to duplicate the Insufficiency of the spleen-qi model. After duplicating the mold 2nd days, distinguish to infuse by corresponding the soup feeding 10 days.Collect the specimen, examining the IL-1 content with Emission immunity method, compare the color method examination D-xylose absorption rate.Use the software processing experiment data of SPSS.Result:1, Fundamental research result :①Chinese medicine classic theories manifestation,The medicine of the Add Medicine Zhizhu soup,87.5% enter the spleen ( stomach) Channels and collaterals,100% can cure the appetite disease;②The modern pharmacology research indicated:Each kind of drugs in the Prescription, regulate the function of the spleen (Digesting system), nervous system, endocrine system, immune system.2, experiment result:The absorption rate of D-xylose about insufficiency of the spleen-qi wistar rat is obviously lower than normal set( P<0.01), recovering normally through treatment,and make no difference with normal set ( P>0.05); The IL-1 contents in serum about insufficiency of the spleen-qi wistar rat is lower than normal set ( P<0.01), recovering normally through treatment,and make no difference with normal set;( P>0.05);The IL-1 contents in brain organization about insufficiency of the spleen-qi wistar rat is higher than normal set ( P<0.05), recovering normally through treatment,and make no difference with normal set ( P>0.05)Conclusion: Add Medicine Zhizhu soup can regulate the content of IL-1 about Insufficiency of the spleen-qi animal, adjust the disorder of stomach and intestines function , raise the small intestines absorption function,elevates the D-xylose absorption rate, repair the harm of the nerve-immunity-endocrine network function, thus attain to cure the insufficiency of the spleen-qi.